| 泰国巨竹试管快繁研究 |
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| 引用本文： | 周玲,王晓芹,林新春.泰国巨竹试管快繁研究[J].浙江农林大学学报,2014,31(5):817-820. |
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| 作者姓名： | 周玲 王晓芹 林新春 |
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| 作者单位： | 浙江农林大学 亚热带森林培育国家重点实验室培育基地, 浙江 临安 311300 |
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| 基金项目： | 国家自然科学基金资助项目(31270677);“十二五”浙江省农业新品种选育“竹木育种协作组”课题(2012C12908-3);浙江省自然科学基金重点资助项目(Z3100366);浙江省优先主题重点农业项目(2010C12011) |
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| 摘 要： | 以泰国巨竹Gigantochloa tekserah成熟种胚为材料, 研究不同激素组合对泰国巨竹丛生芽增殖及生根的影响, 建立其试管繁殖体系。结果表明:泰国巨竹丛生芽的最佳增殖培养基为MS(Murashige and Skoog)+3 mg·L-1 BA(6-苄基腺嘌呤)+0.01 mg·L-1 TDZ (噻苯隆)+0.3 mg·L-1NAA(萘乙酸), 增殖系数为2.11, 芽丛生长旺盛; 最优生根培养基为1/2MS+3.0 mg·L-1 IBA(吲哚丁酸), 生根率达70%, 根系发达、粗壮; 试管苗移栽至等配比的泥炭、蛭石、珍珠岩的基质中, 成活率达100%。
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| 关 键 词： | 植物学 泰国巨竹 组织培养 丛生芽 植物生长调节物质 |
| 收稿时间： | 2013-10-18 |
In vitro micropropagation of Gigantochloa tekserah |
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| ZHOU Ling;WANG Xiaoqin;LIN Xinchun.In vitro micropropagation of Gigantochloa tekserah[J].Journal of Zhejiang A&F University,2014,31(5):817-820. |
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| Authors: | ZHOU Ling;WANG Xiaoqin;LIN Xinchun |
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| Institution: | The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an 311300, Zhejiang, China |
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| Abstract: | To establish a tissue culture system for commercial propagation, buds induced by mature embryos were used as explants to study the effect of different hormone combinations on bud multiplication and root induction of Gigantochloa tekserah. Results showed that for bud multiplication, since the highest proliferation coefficient was 2.11 and cluster buds grew well, Murashige and Skoog's (MS) medium supplemented with 3 mg·L-1 6-benzylaminopurine (BA), 0.01 mg·L-1 thidiazuron (TDZ), and 0.3 mg·L-1 1-naphthylacetic acid (NAA) was the optimal medium. The best rooting medium was 1/2 MS supplemented with 3 mg·L-1 indole-3-butyric acid (IBA) because of the rooting rate was up to 70% with long, thick roots. After transferring to an equal ratio mixture of peat, vermiculite, and perlite, the survival rate of hardening plantlets was 100%. |
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