| 鸭圆环病毒PT07基因组序列测定与分析 |
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| 引用本文： | 施少华,傅光华,程龙飞,陈红梅,万春和,陈珍,彭春香,林芳,黄瑜.鸭圆环病毒PT07基因组序列测定与分析[J].中国预防兽医学报,2010,32(3). |
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| 作者姓名： | 施少华 傅光华 程龙飞 陈红梅 万春和 陈珍 彭春香 林芳 黄瑜 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建,福州350013;福建省畜禽疫病防治工程技术研究中心,福建,福州350013
2. 福建省农业科学院畜牧兽医研究所,福建,福州350013 |
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| 基金项目： | 福建省财政专项-福建省农科院科技创新团队建设基金 |
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| 摘 要： | 本研究采用PCR方法从临床健康的番鸭法氏囊组织中分段扩增获得鸭圆环病毒(DuCV)PT07全长基因组,将扩增片段分别克隆,获得重组质粒,测序结果表明,DuCV基因组全长为1988nt。经基因组结构分析发现,DuCVPT07的基因组有3个开放阅读框(ORF),其中V1/rep和C1/cap是2个主要的ORF,分别编码Rep蛋白和Cap蛋白;在V1/rep和C1/cap的5'起始端之间存在与启动滚环复制有关的1个茎环结构和1个正向重复序列。遗传进化分析发现,DuCV可分为2个群,PT07与TC1/2002～TC4/2002和FJ0604同处一个进化分支,而与Ger、33753-52和MH25关系较远。
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| 关 键 词： | 鸭圆环病毒 基因组 序列分析 |
Sequencing and analysis of genome of duck circovirus PT07 |
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| SHI Shao-hua,FU Guang-hua,CHENG Long-fei,CHEN Hong-mei,WAN Chun-he,CHEN Zhen,PENG Chun-xiang,LIN Fang,HUANG Yu.Sequencing and analysis of genome of duck circovirus PT07[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). |
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| Authors: | SHI Shao-hua FU Guang-hua CHENG Long-fei CHEN Hong-mei WAN Chun-he CHEN Zhen PENG Chun-xiang LIN Fang HUANG Yu |
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| Institution: | SHI Shao-hua1,2,FU Guang-hua1,CHENG Long-fei1,CHEN Hong-mei1,WAN Chun-he1,CHEN Zhen1,PENG Chun-xiang1,LIN Fang1,HUANG Yu1,2 (1. Institute of Animal Husb,ry , Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China,2. Fujian Animal Diseases Control Techology Development Center,China) |
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| Abstract: | A complete genome of duck circovirus (DuCV) was amplified by PCR from bursa of a healthy muscovy duck. The DuCV (designate as PT07) genome is 1 988 nt in length containing three open reading frames (ORFs),of which the two major ORFs V1/rep and C1/cap ORF encode Rep protein and Cap protein respectively. A stem-loop structure and a directly repeat sequence element,which are indicative of a rolling-circle mode of replication,are located at the intergenic regions of the 5'-ends of V1/rep and C1/cap. The duck ci... |
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| Keywords: | duck circovirus genome sequence analysis |
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