Disposition and metabolism of [14C]chlorpyrifos in the black imported fire ant, Solenopsis richteri Forel |
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| Authors: | Janice E Chambers WThomas Redwood Carol A Trevathan |
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| Institution: | Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762 USA |
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| Abstract: | The short-term disposition and metabolism of topically administered 14C]chlorpyrifos was assessed in the black imported fire ant (Solenopsis richteri Forel) in the presence and absence of the mixed-function oxidase inhibitor piperonyl butoxide. Chlorpyrifos is readily absorbed into an internal organosoluble fraction which was quickly converted into a water-soluble fraction. The radioactivity was slowly excreted over a 24-hr period. Piperonyl butoxide slowed the conversion of the internal organosoluble radioactivity to the water-soluble fraction. Thin-layer chromatography indicated that piperonyl butoxide slowed the conversion of chlorpyrifos to material remaining at the origin, presumably water-soluble metabolites. The results of acid hydrolysis studies indicated that the water-soluble radioactivity was comprised mainly of conjugates. Although very little chlorpyrifos oxon was recovered in the metabolism experiments, in vitro studies on fire and head homogenates showed the compound to be an extremely potent anticholinesterase, with an I50 of 4.6 × 10?10M, while a major metabolite, 3,5,6-trichloropyridinol, was an ineffective acetylcholinesterase inhibitor. |
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| Keywords: | To whom all correspondence should be addressed |
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