| PCR-SSCP对我国鲁道夫对盲囊线虫的分子鉴定 |
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| 引用本文： | 陈虹虹,陈晓梅,杨廷宝,何芳,翁亚彪,林瑞庆,朱兴全.PCR-SSCP对我国鲁道夫对盲囊线虫的分子鉴定[J].中国兽医学报,2006,26(1):35-38. |
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| 作者姓名： | 陈虹虹 陈晓梅 杨廷宝 何芳 翁亚彪 林瑞庆 朱兴全 |
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| 作者单位： | 1. 华南农业大学兽医学院,广东,广州,510642
2. 中山大学,生命科学学院,广东,广州,510275 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 对来自青海湖的鲁道夫对盲囊线虫（Contracaecum rudolphii）核糖体DNA第一、第二内转录间隔区（ITS-1、ITS-2）进行PCR扩增、DNA单链构象多态性（SSCP）分析及序列分析。并与来自欧洲的2个姊妹种鲁道夫对盲囊线虫进行了比较。结果显示，我国青海湖的鲁道夫对盲囊线虫与来自意大利的（.rudolphii B具有一样的SSCP带型及ITS序列，但不同于C．rudolphiiA．因此属于C.rudolphii B。本试验证实了ITS片段可作为遗传标记用于鉴别鲁道夫对盲囊线虫的姊妹种，从而为鲁道夫对盲囊线虫的进一步研究奠定了基础。
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| 关 键 词： | 鲁道夫对盲囊线虫 内转录间隔区(ITS) 序列分析 |
| 文章编号： | 1005-4545(2006)01-0035-04 |
| 收稿时间： | 2004-08-02 |
| 修稿时间： | 2004年8月2日 |
Molecular Identification of Contracaecum rudolphii from China by PCR-SSCP |
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| CHEN Hong-hong,CHEN Xiao-mei,YANG Ting-bao,HE Fang,WENG Ya-biao,LIN Rui-qing,ZHU Xing-quan.Molecular Identification of Contracaecum rudolphii from China by PCR-SSCP[J].Chinese Journal of Veterinary Science,2006,26(1):35-38. |
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| Authors: | CHEN Hong-hong CHEN Xiao-mei YANG Ting-bao HE Fang WENG Ya-biao LIN Rui-qing ZHU Xing-quan |
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| Institution: | 1. College of Veterinary Medicine,South China Agricultural University, Guangzhou 510642 ,China ; 2. College of Life Science,Sun Yet-sen University ,Guangzhou 510275 ,China |
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| Abstract: | The aim of the present study was to identify specimens representing Contrancaecum rudolphii which were isolated from Qinghai Lake,China.The first and the second internal transcribed spacers(ITS-1,ITS-2) of ribosomal DNA were amplified by PCR from individual Chinese C.rudolphii and the amplicons were analysed by single-strand conformation polymorphism(SSCP).The results of SSCP analyses revealed that all the Chinese C.rudolphii specimens represented C.rudophiiB.One specimen was sequenced and the ITS-1 and ITS-2 sequences were identical to that of C.rudophii B,consistent with the results of SSCP analysis.For the first time in China,this study demonstrated that ITS-1 and ITS-2 sequences could be applied as genetic markers for the identification of Chinese C.rudolphii,and the findings have implications for studying the systematics and molecular genetics of anisakid nematodes. |
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| Keywords: | PCR-SSCP |
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