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| 鹅卵泡颗粒细胞凋亡及其与生殖激素间的关系 | |
| 引用本文： | 陈秀萍,姜勋平,丁家桐.鹅卵泡颗粒细胞凋亡及其与生殖激素间的关系[J].畜牧兽医学报,2006,37(10):982-986. |
| 作者姓名： | 陈秀萍 姜勋平 丁家桐 |
| 作者单位： | 1. 华中农业大学动物科技学院,武汉,430070;扬州大学动物科学与技术学院,扬州,225009 2. 华中农业大学动物科技学院,武汉,430070 3. 扬州大学动物科学与技术学院,扬州,225009 |
| 基金项目： | 国家自然科学基金项目（30300253）;中国博士后科学基金项目（2003033469） |
| 摘 要： | 采用正处于产蛋期的扬州鹅10只，取其卵泡分离颗粒层细胞，用70％乙醇固定后，应用流式细胞术分析颗粒细胞凋亡情况。同时，用连续采血的方法，每3h采血一次，分离血浆，用放射免疫分析法测定血浆促卵泡素（FSH）、促黄体素（LH）、雌激素（Ez）水平，用以分析激素水平与凋亡间的关系。结果表明：（1）无论是健康卵泡还是闭锁卵泡，颗粒细胞90％以上均处于G1期，2％～6％处于G2期，S期比例最小；（2）闭锁卵泡颗粒细胞凋亡率极显著高于健康卵泡（P〈0．01）；（3）随着健康卵泡直径的增加，颗粒细胞的凋亡呈降低趋势；（4）健康组鹅的FSH、E2水平要高于闭锁组，而LH水平要低于闭锁组。 |
| 关 键 词： | 扬州鹅 细胞凋亡 颗粒细胞 生殖激素 |
| 文章编号： | 0366-6964（2006）10-0982-05 |
| 收稿时间： | 2005-09-08 |
| 修稿时间： | 2005-09-08 |
Apoptosis of Goose Granulosa Cells and Its Relationship between Reproductive Hormones |
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| CHEN Xiu-ping,JIANG Xun-ping,DING Jia-tong.Apoptosis of Goose Granulosa Cells and Its Relationship between Reproductive Hormones[J].Acta Veterinaria et Zootechnica Sinica,2006,37(10):982-986. | |
| Authors: | CHEN Xiu-ping JIANG Xun-ping DING Jia-tong |
| Institution: | 1. College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China ; 2. College of Animal Science and Technology , Yangzhou University, Yangzhou 225009, China |
| Abstract: | Ten laying-egg-yangzhou-gooses were sacrificed to obtain their follicles,from which granulosa cells were collected.After the granulosa cells were separated,they were fixed by 70% ethanol,and the apoptotic cell death was evaluated by flow cytometry.Meanwhile,the plasma samples were collected to evaluate the level of FSH,LH and E_2 by RIA methods.The relationships between hormone contents and cell apoptosis rate were calculated.The results showed that:(1)Whether the follicle was healthy or atresic,the percentage of G_1 phase of granulose cells was more than 90%,about 2%-6% granulosa cells were in G_2 phase,and the percentage of S phase was the lowest;(2) The apoptosis rate of granulosa cells of atresia follicles was extremely significant higher than that of healthy follicles(P<0.01);(3) With the incensement of follicular diameter,the apoptosis rate of healthy follicles decreased correspondingly;(4) The level of FSH,E_2 of healthy group were higher than that of apoptotic group,while the level of LH was on the other hand. |
| Keywords: | Yangzhou goose apoptosis granulosa cells reproductive hormone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |