Characterization of Italian grasspea (<Emphasis Type="Italic">Lathyrus sativus</Emphasis> L.) germplasm using agronomic traits,biochemical and molecular markers |
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| Authors: | Lucia Lioi Francesca Sparvoli Gabriella Sonnante Gaetano Laghetti Francesco Lupo Massimo Zaccardelli |
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| Institution: | (1) CNR-Istituto di Genetica Vegetale, Via Amendola 165/A, 70126 Bari, Italy;(2) CNR-Istituto di Biologia e Biotecnologia Agraria, Via Bassini 15, 20133 Milan, Italy;(3) CRA-Centro di Ricerca per l’ Orticoltura, Azienda Sperimentale di Battipaglia, SS 18 204, 84091 Battipaglia, SA, Italy |
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| Abstract: | Genetic relationships among 13 grasspea (Lathyrus sativus L.) landraces mainly collected in Southern Italy were assessed using agronomic traits, biochemical and molecular markers.
Field trials were carried out in two locations and revealed a high influence of field locations on yield, but a low genotype × environment
interaction. Despite this, the agronomic data obtained provided useful information for the choice of the best grasspea landraces
for southern Italian marginal areas. Seed storage proteins utilised as biochemical markers were not able to detect polymorphisms,
on the contrary both classes of molecular markers used i.e. AFLP and SSR, provided useful information on genetic variation
and relationships among landraces. Even though the number of polymorphic fragments detected by AFLP technique was low, it
was sufficient to discriminate all the accessions. The use of SSR to detect polymorphic sites in grasspea showed that most
landraces were clearly grouped in two sub-clusters. One comprised two landraces from most northern localities, while all the
other landraces were clustered together at a very narrow genetic distance. |
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