| 禽用疫苗病毒浓缩技术的研究 |
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| 引用本文： | 姜北宇,刘月焕,景小冬,黄凤军,校海霞,章振华.禽用疫苗病毒浓缩技术的研究[J].华北农学报,2007,22(5):165-171. |
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| 作者姓名： | 姜北宇 刘月焕 景小冬 黄凤军 校海霞 章振华 |
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| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,北京,100097
2. 中国科学院微生物研究所,北京,100080 |
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| 摘 要： | 采用FILTRON中型盒式超滤系统,分别选用截留分子量为30,50,100及300 K 4种不同孔径的超滤膜,对NDV,AIV,EDS76V,IBV及IBDV共5种病毒抗原液进行了浓缩试验研究。结果显示,这4种孔径的超滤膜均可用于上述5种病毒液的浓缩,其病毒的截留率可达99.9%~100%;采用各种病毒浓缩液、未浓缩病毒液及超滤液制成各种相应浓缩苗、未浓缩苗及超滤液乳剂免疫试验鸡。试验证明,浓缩苗明显优于未浓缩苗,在病毒浓缩过程中均未出现有效抗原成分的丢失,其抗原性也未发生变化。
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| 关 键 词： | 病毒浓缩 疫苗 免疫 鸡 |
| 文章编号： | 1000-7091（2007）05-0165-07 |
| 修稿时间： | 2006-04-03 |
Studies on Concentration of Avian Viruses Used in Poultry Vaccines |
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| JIANG Bei-yu,LIU Yue-huan,JING Xiao-dong,HUANG Feng-jun,XIAO Hai-xia,ZHANG Zhen-hua.Studies on Concentration of Avian Viruses Used in Poultry Vaccines[J].Acta Agriculturae Boreali-Sinica,2007,22(5):165-171. |
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| Authors: | JIANG Bei-yu LIU Yue-huan JING Xiao-dong HUANG Feng-jun XIAO Hai-xia ZHANG Zhen-hua |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary Science, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China;2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | By using the FILTRON medium-cassette ultra-filtration system,the viral antigens of newcastle disease virus(NDV),avian influenza virus(AIV),infectious bronchitis virus(IBV),infectious bursal disease virus(IBDV) and egg drop syndrome virus(EDS76V) were concentrated with different pore size ultra-filtration membranes of 30,50,100 and 300 K respectively.The results showed that the above five viruses could be concentrated by 30,50,100 and 300 K molecular weight of ultra-filtration membranes,the virus recovery rate reached 99.90%-100%.The oil emulsion vaccines were made with concentrated virus fluid,virus fluid and ultra-filtrated fluid,and then were tested in chickens.The vaccination results revealed that concentrated virus vaccines were better than original virus vaccines.The effectiveness of virus fluid were not lost in the course of viral concentration and the virus antigenicity were not altered either. |
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| Keywords: | Virus concentration Vaccines Vaccination Chicken |
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