| 大樱桃砧木吉塞拉组培快繁技术研究 |
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| 引用本文： | 张玉君,彭兴龙,张哲民.大樱桃砧木吉塞拉组培快繁技术研究[J].安徽农业科学,2009,37(10):4384-4385. |
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| 作者姓名： | 张玉君 彭兴龙 张哲民 |
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| 作者单位： | 河南省三门峡市林业技术推广站,河南三门峡,472000;河南省灵宝市鼎原樱桃专业合作社,河南灵宝,472542 |
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| 摘 要： | 目的]建立大樱桃砧木吉塞拉组培快繁技术体系。方法]以吉塞拉5号的茎尖分生组织为外植体,从材料丛生芽的诱导、继代、生根以及炼苗与移栽方面研究其组培快繁技术。结果]结果表明,适宜丛生芽诱导的培养基为MS＋6-BA 1.00 mg/L＋NAA 0.03mg/L＋KT 1.50 mg/L＋GA30.20 mg/L;试管苗在以1/2 MS＋IBA 1.50 mg/L的培养基上生根效果好;以1/2草粪＋1/2园土为移栽基质时,小苗成活率高。结论]为大规模、快速生产吉塞拉组培苗提供理论和实践依据。
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| 关 键 词： | 大樱桃 砧木 吉塞拉 组织培养 |
Study on Tissue Culture and Rapid Propagation Technology of Big Sweet Cherry Rootstock Gisela |
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| ZHANG Yu-jun et al.Study on Tissue Culture and Rapid Propagation Technology of Big Sweet Cherry Rootstock Gisela[J].Journal of Anhui Agricultural Sciences,2009,37(10):4384-4385. |
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| Authors: | ZHANG Yu-jun |
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| Institution: | ZHANG Yu-jun et al(Forestry Science Popularize Station,Sanmenxia,Henan 472000) |
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| Abstract: | Objective] The research aimed to establish the tissue culture and rapid propagation technology system of big sweet cherry rootstock Gisela.Method]With the shoot apical meristem of Gisela No.5 as material,the paper studied the tissue culture and rapid propagation technology from axillary buds induction,subculture,rooting and plantlet hardening and transplantation.Result]The results showed that the optimum medium for axillary buds induction was MS+6-BA 1.00 mg/L +NAA 0.03 mg/L +KT 1.50 mg/L +GA3 0.20 mg/L;... |
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| Keywords: | Big sweet cherry Rootstock Gisela Tissue culture |
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