| 美国红枫外植体选择及启动培养研究 |
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| 引用本文： | 李莹,罗晓芳,蒋湘宁.美国红枫外植体选择及启动培养研究[J].黑龙江农业科学,2010(8):6-9. |
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| 作者姓名： | 李莹 罗晓芳 蒋湘宁 |
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| 作者单位： | 北京林业大学,生物科学与技术学院,北京,100083 |
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| 摘 要： | 对美国红枫组织培养中外植体灭菌、褐化预防及外植体启动进行了试验研究。结果表明:以美国红枫半木质化茎段为外植体,用1%次氯酸钠灭菌4 min,外植体的成活率最高为82.22%;在外植体培养初期每隔2 d转移至新鲜培养基中,2~3次后可以彻底去除褐化现象;最佳的启动培养基为MS+6-BA 1.0 mg.L-1+NAA 0.1 mg.L-1,外植体抽芽率可达92.2%。
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| 关 键 词： | 美国红枫 外植体 消毒剂 褐化 组织培养 |
Selection of Explant and Initial Culture of America Red Maple in Tissue Culture |
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| LI Ying,LUO Xiao-fang,JIANG Xiang-ning.Selection of Explant and Initial Culture of America Red Maple in Tissue Culture[J].Heilongjiang Agricultural Science,2010(8):6-9. |
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| Authors: | LI Ying LUO Xiao-fang JIANG Xiang-ning |
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| Institution: | (Biological Sciences and Biotechnology College of Beijing Forestry University, Beijing 100083) |
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| Abstract: | The America Red Maple（Acer rubrurn）was taken as material cultured in vitro to study sterilization techniques,browning prevention and explants initial culture. The results showed that using haif-lignification stems as explants, the highest survival rate of the explants was 82.22 % after the explant were sterilized in 1% NaClO for 4 min. Transfer explants into fresh medium every 2 d at primary stage,browning can be eliminated then after 2-3 times. The MS medium containing 1.0 mg·L^-1 6-BA and 0.1 mg·L^-1 NAA was most efficient in the initial culture of Acer palmatum atropurpureum,sprouting rate of explants was 92.2%. |
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| Keywords: | Acer rubrurn explants disinfectors browning tissue culture |
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