Expression of Putative Escherichia coli Heat-labile Enterotoxin (LT) Receptors on Intestinal Brush Borders from Pigs of Different Ages |
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| Authors: | P A Grange L A Parrish A K Erickson |
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| Institution: | (1) Veterinary Science Department, South Dakota State University, N. Campus Drive, PO Box 2175, USA;(2) Biology/Microbiology Department, South Dakota State University, Brookings, SD 57007-1396, USA |
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| Abstract: | Many strains of enterotoxigenic Escherichia coli (ETEC) that cause diarrhoea in young piglets secrete a heat-labile enterotoxin (LTp) that binds to specific glycoconjugates
on porcine intestinal epithelial cells. Binding of LTp to an appropriate glycoconjugate facilitates the uptake and trafficking
of the toxin into the cell, where it stimulates intracellular changes that promote fluid secretion and diarrhoea. The objective
of the current study was to identify the LTp-binding glycoconjugates on porcine intestinal epithelial cells, the natural target
cells for LTp. We found that LTp binds, in an age-correlated manner, to an acidic glycosphingolipid (GSL) that co-migrated
with GM1 on thin-layer chromatography (TLC), a small acidic GSL that appears to be a sulphatide, a neutral GSL that co-migrated
with neolactotetraglycosylceramide (nLc4) on TLC, and two glycoproteins (36 and 205 kDa). Of these potential LTp receptors,
the GM1-co-migrating GSL was detected most intensely in young animals, while the other four LTp-binding glycoconjugates were
detected most intensely in older pigs (54 weeks). Since ETEC primarily cause disease in young piglets, the GM1-co-migrating
GSL is the most likely candidate for a functional LTp receptor. |
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| Keywords: | enterotoxigenic enterotoxin Escherichia coli heat-labile toxin LTp pig intestinal receptor |
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