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| 鸭疫里默氏菌环介导等温扩增检测方法的建立 | |
| 引用本文： | 陈红梅 程龙飞,施少华,傅光华,万春和,黄 瑜.鸭疫里默氏菌环介导等温扩增检测方法的建立[J].中国农学通报,2010,26(20):15-17. |
| 作者姓名： | 陈红梅 程龙飞 施少华 傅光华 万春和 黄 瑜 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013 2. 福清出入境检验检疫局,福建福清,350300 |
| 基金项目： | 鸭传染性荚膜炎与大肠杆菌病快速鉴别诊断技术的建立与应用 |
| 摘 要： | 根据鸭疫里默氏菌(Riemerella anatipestifer，RA) ompA基因设计一套引物，同时优化其反应条件，建立鸭疫里默氏菌感染的环介导等温扩增 (LAMP）快速检测方法。结果表明，LAMP法的最佳反应条件为反应温度65℃，反应时间80 min。 |
| 关 键 词： | 王草 柱花草 杂交水牛 育肥 王草 柱花草 杂交水牛 育肥 |
| 收稿时间： | 2010/5/19 0:00:00 |
| 修稿时间： | 2010/6/21 0:00:00 |
Rapid Detection of Riemerella anatipestifer infection by Loop-Mediated Isothermal Amplification Assay |
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| Chen Hongmei,Cheng Longfei,Shi Shaohua,Fu Guanghua,Wan Chunhe,Huang Yu,Li Songyu.Rapid Detection of Riemerella anatipestifer infection by Loop-Mediated Isothermal Amplification Assay[J].Chinese Agricultural Science Bulletin,2010,26(20):15-17. | |
| Authors: | Chen Hongmei Cheng Longfei Shi Shaohua Fu Guanghua Wan Chunhe Huang Yu Li Songyu |
| Institution: | Chen Hongmei1,Cheng Longfei1,Shi Shaohua1,Fu Guanghua1,Wan Chunhe1,Huang Yu1,Li Songyu2 (1Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,2Fuqing Entry-Exit Inspection and Quarantine Bureau of the People Republic of China,Fuqing 350300) |
| Abstract: | In order to detect Riemerella anatipestifer rapidly,a set of specific primers based on the RA ompA gene was designed and synthesized to amplify the special DNA sequences by LAMP. Meanwhile,the reaction conditions of LAMP were optimized,sensitivity and specificity were tested. The results showed that the best amplification condition was 65℃ for 80 min. The LAMP method developed in this experiment was of high specificity and sensitivity. |
| Keywords: | Riemerella anatipestifer loop-mediated isothermal amplification rapid detection |
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