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| 农杆菌介导轮状病毒抗原蛋白基因VP7转化紫花苜蓿的研究 | |
| 引用本文： | 曲静,王丕武,关淑艳,范玉广.农杆菌介导轮状病毒抗原蛋白基因VP7转化紫花苜蓿的研究[J].中国草地学报,2011,33(5). |
| 作者姓名： | 曲静 王丕武 关淑艳 范玉广 |
| 作者单位： | 1. 吉林农业大学生命科学院,吉林长春,130118 2. 吉林农业大学农学院,吉林长春,130118 3. 吉林省育华药业有限责任公司,吉林长春,130000 |
| 基金项目： | 吉林省科技厅项目“甜菜碱合成酶基因转化羊草研究”(20040540) |
| 摘 要： | 以公农1号紫花苜蓿5～7日龄无菌苗子叶为材料,通过农杆菌介导法将轮状病毒抗原蛋白基因VP7转入紫花苜蓿子叶中,并用卡那霉素进行筛选获得抗性植株.提取转化抗性植株叶片DNA作模板,用VP7基因特异引物进行PCR扩增,琼脂糖凝胶电泳分析表明有7株扩增出了960bp的特异条带.对7株PCR阳性植株进行PCR- Southern杂交和Southern杂交分析,有4株出现阳性杂交信号,表明目的基因已经整合到紫花苜蓿基因组中,并且是1～2个拷贝. |
| 关 键 词： | 紫花苜蓿 子叶 VP7基因 转化 |
Research on Transforming Antigen Gene VP7 into Alfalfa with Agrobacterium-mediated Method |
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| QU Jing,WANG Pi-wu,GUAN Shu-yan,FAN Yu-guang.Research on Transforming Antigen Gene VP7 into Alfalfa with Agrobacterium-mediated Method[J].Chinese Journal of Grassland,2011,33(5). | |
| Authors: | QU Jing WANG Pi-wu GUAN Shu-yan FAN Yu-guang |
| Institution: | QU Jing1,WANG Pi-wu2,GUAN Shu-yan1,FAN Yu-guang3(1.College of Life Science,Jilin Agricultural University,Changchun 130118,China,2.College ofAgronomy,3.Jilin Province YuhuaPharmaceutical Industry Company,Lted,Changchun 130000,China) |
| Abstract: | 5~7 days old aseptic seedling of Medicago sativa cv.Gongnong No.1 was used as material.The cotyledons of Gongnong No.1 were selected as explants for genetic transformation,Antigen Gene VP7 was transformed into Medicago sativa cv.Gongnong No.1 with Agrobacterium-mediated method.And individuals of resistance were obtained in the selection medium supplemented with kanamycin.The genomic DNA in leaf of all transgenic plants was extracted as template DNA,gene VP7 was amplified with peculiar primer,and peculiar ba... |
| Keywords: | Alfalfa Cotyledons Gene VP7 Transformation |
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