| 不同外植体对多年生黑麦草愈伤组织诱导的影响 |
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| 引用本文： | 陈季琴,韩烈保,杨纯奇,李雪.不同外植体对多年生黑麦草愈伤组织诱导的影响[J].草原与草坪,2005(4):42-46,50. |
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| 作者姓名： | 陈季琴 韩烈保 杨纯奇 李雪 |
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| 作者单位： | 北京林业大学,草坪研究所,北京,100083;湖北鄂州大学,成教处,湖北,鄂州,436000 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 对多年生黑麦草的3个品种进行愈伤组织诱导,选取成熟种子、成熟胚、胚轴、胚根4种外植体。结果表明,胚轴、胚根诱导效果较差,成熟胚在愈伤组织的诱导率、鲜重及愈伤质量方面均优于成熟种子。2,4-D浓度在2~4mg/L,6-BA浓度为0.025mg/L时有利于成熟胚诱导产生愈伤组织;成熟种子为外植体时,2,4-D浓度在5~8mg/L,6-BA浓度在0.025mg/L或0.2mg/L有利于成熟种子诱导产生愈伤组织。2,4-D浓度为2mg/L,6-BA浓度为0.5mg/L时成熟胚和成熟种子诱导的愈伤组织鲜重均达到最大。特拉华在愈伤组织的诱导率、增殖速度及愈伤质量方面表现较优,托亚次之,尤文图斯较差。AC和LH有利于植株的再生。
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| 关 键 词： | 多年生黑麦草 外植体 成熟种子 成熟胚 愈伤组织诱导 |
| 文章编号： | 1009-5500（2005）04-0042-05 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2004-11-11 |
Effect of different explants on callus induction of perennial ryegrass |
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| CHEN Ji-qin,HAN Lie-bao,YANG Chun-qi,LI Xue.Effect of different explants on callus induction of perennial ryegrass[J].Grassland and Turf,2005(4):42-46,50. |
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| Authors: | CHEN Ji-qin HAN Lie-bao YANG Chun-qi LI Xue |
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| Abstract: | Three perennial ryegrass (Lolium perenne) varieties were cultured for callus induction on MS basic medium containing different concentration of 2,4-D and 6-BA by using different explants. The result showed that hypocotyls and radicels were ineffective in callus induction; 24 mg/L 2,4-D with 0.025 mg/L 6-BA were propitious to callus induction from mature embryos; 58 mg/L 2,4-D with 0.025 mg/L or 0.2mg/L 6-BA were propitious to callus induction from mature seeds; the fresh weight of callus reaches the largest with 2 mg/L 2,4-D and 0.5 mg/L 6-BA of the three varieties. Delaware proved to be the best of the three, comes Taya and Juventus is the worst. AC and LH are effective in shoot regeneration. |
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| Keywords: | perennial ryegrass explant mature seed mature embryos callus induction |
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