| 纤维素降解菌筛选分离与CMC酶提取及其活性研究 |
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| 引用本文： | 聂麦茜,刘加昌,朱玉玺,李立伟,田永胜.纤维素降解菌筛选分离与CMC酶提取及其活性研究[J].安徽农业科学,2008,36(19). |
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| 作者姓名： | 聂麦茜 刘加昌 朱玉玺 李立伟 田永胜 |
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| 作者单位： | 西安建筑科技大学环境与市政工程学院,陕西西安,710055 |
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| 摘 要： | 目的]研究纤维素酶的分离、筛选及产酶条件优化。方法]从常年堆积秸秆下的腐殖土、造纸废液死水下的污泥、屠宰场的新鲜牛胃和牛肠及其温热的牛粪等中大量取样,通过刚果红筛选培养基、滤纸观察培养基、滤纸失重率初筛与纤维素酶活力复筛,筛选出4株纤维素高效降解菌A1、A8、B4、C2,对A1菌和C2菌进行了鉴定,并研究其发酵麦秸秆产CMC酶的提取条件及酶活性和稳定性。结果]试验结果表明,A1菌与C2菌2株菌产酶的最佳条件基本相似,其粗酶提取利用浓度2%的NaCl溶液、固液比为1∶15时较经济合理,pH值为5.0,50~60℃范围内CMC酶的活性及稳定性较高。结论]纤维素酶促反应无需进行大规模搅拌。
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| 关 键 词： | 纤维素 优良菌 筛选分离 酶 |
Study on the Screening,Isolation of Cellulose Decomposition Microorganisms and Extraction,Activity of CMCase |
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| NIE Mai-qian et al.Study on the Screening,Isolation of Cellulose Decomposition Microorganisms and Extraction,Activity of CMCase[J].Journal of Anhui Agricultural Sciences,2008,36(19). |
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| Authors: | NIE Mai-qian |
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| Abstract: | Objective]The aim of the research was to study the screening,isolation of cellulose decomposition microorganisms and the optimization of enzyme-producing.Method] Samples with efficient degradation ability for cellulose were isolated from soil beneath stalk-stacking,sludge beneath stagnant papermaking effluent and fresh bovine stomach,intestine,warmed manure.After measured the hydrolyzed circle caused on plates of congo red dying,determined the rottrn change of filter paper,primary screened with lost rate of filter weightness,secondary screened of CMCase activity measurement,four strains A1,A8,B4 and C2 were screened out.A1 and C2 was identified as Pseudomonas and Trichoderma viride respectively.Result] The experimental results showed that the CMCase production conditions of A1 and C2 were similar.2% NaCl and the ratio of solid to liquid 1∶15 was economical and reasonable for the extraction of CMCase from solid cultural broth.The enzyme could maintain activity under the condition of 50-60 ℃,pH value 5.0.Conclusion] Gently agitation was necessary for the production of CMCase. |
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| Keywords: | Cellulose Preponderant strains Screening and separating Enzyme |
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