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等氮量分次追施对盆栽红富士苹果叶片衰老及15N-尿素吸收、利用特性的影响
引用本文:丁宁,姜远茂,彭福田,陈倩,王富林,周恩达.等氮量分次追施对盆栽红富士苹果叶片衰老及15N-尿素吸收、利用特性的影响[J].中国农业科学,2012,45(19):4025-4031.
作者姓名:丁宁  姜远茂  彭福田  陈倩  王富林  周恩达
作者单位:1.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018
基金项目:现代农业产业技术体系建设专项资金(CARS-28);国家公益性行业(农业)科研专项资金(201103003);山东省农业重大应用创新课题(201009)
摘    要:【目的】为苹果生产中确定延缓叶片衰老的追肥策略提供依据。【方法】以两年生盆栽红富士苹果嫁接苗/平邑甜茶为试材,研究等氮量分次追施氮肥对红富士苹果叶片衰老的影响及15N-尿素的吸收利用特性。【结果】不同追肥处理,植株叶片叶面积、叶绿素含量和叶片全氮含量在盛花期均以一次性追肥处理最高,三次追肥处理最低,且与一次追肥处理差异显著;在花芽分化期均以二次追肥处理最高,一次追肥处理最低;在果实采收期均以三次追肥处理最高,一次追肥处理最低,且与三次追肥处理差异显著;叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性在盛花期均以一次性追肥处理最高,三次追肥处理最低,且与一次追肥处理差异显著;在花芽分化期均以二次追肥处理最高,一次追肥处理最低;在果实成熟期均以三次追肥处理最高,一次追肥处理最低,且与三次追肥处理差异显著;果实成熟期植株各器官Ndff值差异显著,三次追肥处理显著高于一次和二次追肥处理;果实成熟期三次追肥处理植株的总氮量、吸收的15N量及15N肥料利用率均为最大。【结论】三次追肥处理能增强苹果叶片对氮的吸收调节能力,延缓生长后期叶片衰老,提高氮肥利用率。

关 键 词:苹果  分次追肥  叶片衰老  15N-尿素  吸收  利用  
收稿时间:2012-05-21

Effect of Topdressing Nitrogen Application on Leaf Senescence and 15 N-Urea of Absorption,Distribution and Utilization in Potted ‘Fuji ’Apple
DING Ning,JIANG Yuan-mao,PENG Fu-tian,CHEN Qian,WANG Fu-lin,ZHOU En-da.Effect of Topdressing Nitrogen Application on Leaf Senescence and 15 N-Urea of Absorption,Distribution and Utilization in Potted ‘Fuji ’Apple[J].Scientia Agricultura Sinica,2012,45(19):4025-4031.
Authors:DING Ning  JIANG Yuan-mao  PENG Fu-tian  CHEN Qian  WANG Fu-lin  ZHOU En-da
Institution:(College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,Shandong)
Abstract:【Objective】The aim of the experiment is to provide a theoretical basis for topdressing application in apple for delaying the leaf senescence.【Method】The effects on leaf senescence and the characteristics of absorption and utilization of potted two-year-old ‘Fuji’ apple/Malus hupenhensis in response to topdressing nitrogen were studied.【Result】Plant leaf area, SPAD and the total N content of leaves were obviously the highest in one-time topdressing application at full-bloom stage, and the lowest were found in three-time topdressing application. At flower bud differentiation stage, two-time topdressing application was the highest, and the lowest was in one-time topdressing application. At fruit maturity stage, three-time topdressing application were obviously the highest, while one-time topdressing application was the lowest. The leaf SOD, POD and CAT activities were obviously the highest in one-time topdressing application at full-bloom stage, the lowest were found in three-time topdressing application. At flower bud differentiation stage, two-time topdressing application was the highest, the lowest was in one-time topdressing application. At fruit maturity stage, three-time topdressing application was obviously the highest, while one-time topdressing application was the lowest. The Ndff value in different organs was significantly different and the value of three-time topdressing application was obviously higher than that of one or two-time topdressing at fruit maturity stage. Plant total nitrogen, 15N uptake and nitrogen ratio were the highest in three-time topdressing application fruit maturity stage.【Conclusion】Three-time topdressing application not only enhanced the absorption and transfer ability to N in apple tree, delayed leaf senescence, but also increased the nitrogen efficiency.
Keywords:apple  topdressing application  leaf senescence  15N-urea  absorption  utilization
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