| 甘蓝抗黑腐病离体鉴定方法的研究 |
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| 引用本文： | 黄德芬,李成琼,司军,任雪松,宋洪元.甘蓝抗黑腐病离体鉴定方法的研究[J].西南农业大学学报,2011,33(8). |
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| 作者姓名： | 黄德芬 李成琼 司军 任雪松 宋洪元 |
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| 作者单位： | 西南大学园艺园林学院重庆市蔬菜学重点实验室教育部南方山地园艺学重点实验室; |
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| 基金项目： | 重庆市科技攻关项目(CSTC,2010AA1033); 农业科技成果转化资金项目(2009F10010355); 中央高校基本科研业务费资金项目(XDJK2010C068,XDJK2010C069)资助 |
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| 摘 要： | 以活体喷雾法作对照,研究了不同离体接种方法、不同接种浓度以及不同苗龄对甘蓝抗病性鉴定的影响.结果表明:采用环叶打孔离体叶片滴接法在甘蓝4～6片真叶期时,将浓度为1.0×108 cfu/mL的细菌悬浮液接种在直径1.5cm甘蓝叶片上,放到铺有湿滤纸的培养皿中密封后在28℃培养箱中培养5d后进行鉴定,其鉴定结果与活体喷雾法鉴定结果一致,可用于抗源筛选、品种(系)抗性鉴定及在抗病突变体筛选等方面.
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| 关 键 词： | 甘蓝 黑腐病 抗性 离体鉴定 |
Studies on the Methods of in vitro Evaluation of Black Rot-Resistance in Cabbage |
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| HUANG De-fen,LI Cheng-qiong,SI Jun,REN Xue-song,SONG Hong-yuan School of Horticulture , L,scape Architecture,Southwest University,Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing ,China.Studies on the Methods of in vitro Evaluation of Black Rot-Resistance in Cabbage[J].Journal of Southwest Agricultural University,2011,33(8). |
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| Authors: | HUANG De-fen LI Cheng-qiong SI Jun REN Xue-song SONG Hong-yuan School of Horticulture L scape Architecture Southwest University Chongqing Key Laboratory of Olericulture Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Chongqing China |
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| Institution: | HUANG De-fen,LI Cheng-qiong,SI Jun,REN Xue-song,SONG Hong-yuan School of Horticulture and Landscape Architecture,Southwest University,Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing 400716,China |
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| Abstract: | |
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| Keywords: | cabbage black rot resistance in vitro evaluation |
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