| 己烯雌酚对幼年香猪睾丸肥大细胞数量的影响 |
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| 引用本文： | 田兴贵,李江森,主性,刘若余.己烯雌酚对幼年香猪睾丸肥大细胞数量的影响[J].西南农业大学学报,2011,33(8). |
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| 作者姓名： | 田兴贵 李江森 主性 刘若余 |
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| 作者单位： | 贵州大学动物科学学院;集美大学水产学院; |
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| 基金项目： | 贵州省自然科学基金资助项目(黔科合J字[2006]2031号); 贵州大学博士基金资助项目(2005) |
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| 摘 要： | 为研究己烯雌酚(Diethylstilbestrol,DES)对幼年雄性香猪睾丸肥大细胞数量的影响,随机将20只20～25日龄的幼年雄性香猪分成4组(1个对照组和3个实验组),对照组只注射生理盐水,每个实验组连续9d经腹腔分别注射不同剂量的己烯雌酚(0.03mg/kg,0.3mg/kg和3mg/kg)后,外科手术取出左侧睾丸并经卡洛氏液固定20h,石蜡包埋后制成5μm组织切片,用改良甲苯胺蓝染色法(MTB)观察己烯雌酚(DES)对幼年香猪睾丸肥大细胞数量的影响.结果表明:与对照组相比,不同剂量的DES都具有促进睾丸和附睾中肥大细胞增多的作用,且具有剂量相关性.但相同剂量的DES对睾丸白膜和间质,附睾头、附睾体和附睾尾中肥大细胞数量的作用效果不同,说明己烯雌酚对肥大细胞数量的影响存在组织差异性,且与剂量有关.
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| 关 键 词： | 己烯雌酚 香猪 睾丸 肥大细胞 |
Effect of DES on the Number of Mast Cells in the Testis of Immature Xiang Pig |
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| TIAN Xing-gui,LI Jiang-sen,ZHU Xing,LIU Ruo-yu .College of Animal Science,Guizhou University,Guiyang ,China,.Fisheries College of Jimei University,Xiamen Fujian.Effect of DES on the Number of Mast Cells in the Testis of Immature Xiang Pig[J].Journal of Southwest Agricultural University,2011,33(8). |
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| Authors: | TIAN Xing-gui LI Jiang-sen ZHU Xing LIU Ruo-yu College of Animal Science Guizhou University Guiyang China Fisheries College of Jimei University Xiamen Fujian |
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| Institution: | TIAN Xing-gui1,LI Jiang-sen1,2,ZHU Xing1,LIU Ruo-yu1 1.College of Animal Science,Guizhou University,Guiyang 550025,China,2.Fisheries College of Jimei University,Xiamen Fujian 361021 |
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| Abstract: | To determine the effects of diethylstilbestrol(DES) exposure on mast cell number of immature Guizhou Xiang pigs,20 male Xiang pigs(20-25 days old) were divided into four groups,one control group and three test groups,with 5 pigs in each.The test groups were intraperitoneally injected with DES at a dose of 0.03 mg/kg,0.3 mg/kg and 3 mg/kg,respectively,for 9 successive days.Then the left testis was removed and fixed in Carlo's solution for 20 h and paraffin-embedded.Five-micron-thick sections were cut for mas... |
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| Keywords: | diethylstilbestrol Xiang pig testis mast cell |
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