| 南果梨果实糖积累过程中相关酶的作用 |
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| 引用本文： | 于年文,李俊才,王家珍,蔡忠民,沙守峰,李宏军.南果梨果实糖积累过程中相关酶的作用[J].西南农业学报,2011,24(3). |
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| 作者姓名： | 于年文 李俊才 王家珍 蔡忠民 沙守峰 李宏军 |
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| 作者单位： | 辽宁省果树科学研究所，辽宁熊岳，115009 |
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| 基金项目： | 国家梨产业技术体系扶持项目(nycytx-29-33) |
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| 摘 要： | 以南果梨为试材,测定了不同发育时期果实中的果糖、葡萄糖、蔗糖和淀粉含量以及相关酶的活性-酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)和淀粉酶的活性,并对果实中糖积累与酶活性的关系进行了分析。结果表明:南果梨果实发育呈单S曲线;幼果期和迅速生长中期,果糖和葡萄糖积累较多,蔗糖无积累,与此期高活性的蔗糖转化酶有关;酸性转化酶、中性转化酶前期活性高,后期活性低;蔗糖合成酶和蔗糖磷酸合成酶的活性变化相似,呈V形变化趋势。果实成熟前蔗糖开始快速积累,蔗糖的积累是多种酶共同作用的结果,酸性转化酶、中性转化酶的活性降低是蔗糖积累的必要前提,当酸性转化酶、中性转化酶活性的消失和SS、SPS活性升高时,开始积累蔗糖;淀粉酶的活性随着果实的迅速生长而上升,果实成熟前活性开始下降。
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| 关 键 词： | 南果梨 糖 转化酶 蔗糖合成酶 蔗糖磷酸合成酶 淀粉酶 |
Roles of Related Enzymes in Accumulation of Sugars in Nanguoli Pear Fruit |
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| YU Nian-wen,LI Jun-cai,WANG Jia-zhen,CAI Zhong-min,SHA Shou-feng,LI Hong-jun.Roles of Related Enzymes in Accumulation of Sugars in Nanguoli Pear Fruit[J].Southwest China Journal of Agricultural Sciences,2011,24(3). |
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| Authors: | YU Nian-wen LI Jun-cai WANG Jia-zhen CAI Zhong-min SHA Shou-feng LI Hong-jun |
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| Institution: | YU Nian-wen,LI Jun-cai,WANG Jia-zhen,CAI Zhong-min,SHA Shou-feng,LI Hong-jun(Liaoning Province Institute of Pomology,Liaoning Xiongyue 115009,China) |
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| Abstract: | Using Nanguoli pear as experiment materials,the content of fructose,glucose,sucrose and the activities of related enzymes,including acid invertase(AI),neutral invertase(NI),sucrose synthase(SS),sucrose phosphate synthase(SPS) and amylase during fruit process were measured,and the correlation between the sugar accumulation and the enzymes activities was analyzed in the fruit of Nanguoli pear.The results showed that the Nanguoli pear fruit growth was in a sigmoid curve.Fructose and glucose were accumulated sh... |
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| Keywords: | Nanguoli pear Sugar Inveratse Sucrose synthase Sucrose phosphate synthase Amylase |
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