| 红掌胶胞炭疽菌的分子检测 |
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| 引用本文： | 邢红梅,丁平,周晓云,王克荣.红掌胶胞炭疽菌的分子检测[J].植物病理学报,2008,38(2):113-119. |
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| 作者姓名： | 邢红梅 丁平 周晓云 王克荣 |
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| 作者单位： | 1. 南京农业大学植物保护学院农业部病虫检测与治理重点实验室, 南京 210095;2. 广州市花卉研究中心, 广州 510360 |
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| 摘 要： | 胶胞炭疽菌是引起红掌炭疽病的病原菌。根据GenBank中炭疽属不同种的ITS序列差异,设计了胶胞炭疽菌的特异性引物E1/E2,由此建立的PCR检测体系可以从38个胶胞炭疽菌菌株中扩增得到329 bp的特异性条带,而扩增其它近似或相关菌株时没有相应的特异性条带。该检测体系对胶胞炭疽菌基因组DNA的扩增灵敏度达到10 pg。将引物E1/E2与ITS区通用引物进行套式PCR扩增后,检测灵敏度至少提高10 000倍。当土中胶胞炭疽菌分生孢子达到200个/g土时可检测出。进一步利用此检测体系对携带病原菌的灌溉水、发病组织进行检测,均能快速稳定地检测出病原菌。
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| 关 键 词： | 胶胞炭疽菌 红掌 分子检测 套式PCR |
Molecular detection of Colletotrichum gloeosporioides in Anthurium andraeanum |
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| XING Hong-mei,DING Ping,ZHOU Xiao-yun,WANG Ke-rong.Molecular detection of Colletotrichum gloeosporioides in Anthurium andraeanum[J].Acta Phytopathologica Sinica,2008,38(2):113-119. |
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| Authors: | XING Hong-mei DING Ping ZHOU Xiao-yun WANG Ke-rong |
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| Institution: | 1. Key Lab of Monitoring and Management of Plant Disease and Insects of Chinese Ministry of Agriculture, Department of Plant Protection, Nanjing Agriculture University, Nanjing 210095, China;2. Guangzhou Flower Research Center, Guangzhou 510360, China |
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| Abstract: | Colletotrichum gloeosporioides caused anthracnose in Anthurium andraeanum.Based on differences in internal transcribed space(ITS) sequences of Colletotrichum genus,a pair of species-specific primers,E1 and E2,was synthesized.The primers amplified a single PCR band of 329 bp with DNA extracted from C.gloeosporioides isolated from A.andraeanum,while other relative strains had no corresponding band.The detection sensitivity was 10 pg of genomic DNA.Using ITS1/ITS4 as the first round primers and E1/E2 as the second round primers,the detection sensitivity increased 10 000-fold to 10 fg.The detection sensitivity for the soil pathogens was 200 conidia/g soil.The PCR-based method developed here could stably and quickly detect the pathogen from water samples and diseased plant. |
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| Keywords: | Colletotrichum gloeosporioides Anthurium andraeanum molecular detection nest PCR |
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