Growth,chlorophyll fluorescence and biochemical markers in clonal ramets of shisham (<Emphasis Type="Italic">Dalbergia sissoo</Emphasis> Roxb.) at nursery stage |
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| Authors: | Azamal Husen |
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| Institution: | (1) Department of Applied Biology, Faculty of Applied Natural Sciences, University of Gondar, P. O. Box 196, Gondar, Ethiopia |
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| Abstract: | About 19 clones of shisham (Dalbergia sissoo Roxb.) were raised by using healthy coppice shoot cuttings. After 2 year of establishment and growth, they were examined
to identify suitable clone of shisham at nursery stage. Clones were studied for growth (height and basal diameter), chlorophyll
fluorescence (Fv/Fm) and biochemical contents (chlorophyll a, b, total chlorophyll, free amino acids, total soluble proteins,
total soluble sugars, starch and reducing sugar) in the leaves. Clone C2 and C3 (Gonda, Uttar Pradesh, India) had the highest
growth and the chlorophyll fluorescence rate among examined clones. Between these two clones, C2 gave the highest response
on height, basal diameter and Fv/Fm. Except free amino acids, all other biochemical contents were greater in the clones belonging
to Gonda, Uttar Pradesh, India. Growth parameters, i.e. height and basal diameter increment at nursery stage had a positive
correlation with all the studied parameters, except free amino acid and total soluble protein. These findings have demonstrated
that there are remarkable clonal variations in growth, physiological and biochemical responses at nursery stage. Thus, physiological
and biochemical markers can enhance selection efficiency, in addition to growth characteristics such as height and basal diameter
as these have practical value in the tree improvement. The study suggests that clones of Gonda, Uttar Pradesh, India had the
best performance and could be further tested in plantation programs. |
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| Keywords: | Clonal ramets Growth Chlorophyll fluorescence Biochemical contents Dalbergia sissoo Tree improvement Evaluation Selection |
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