| 应用RAPD标记鉴定苦瓜杂种纯度 |
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| 引用本文： | 林珲,李永平,朱海生,李大忠.应用RAPD标记鉴定苦瓜杂种纯度[J].福建农业学报,2011,26(6):977-980. |
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| 作者姓名： | 林珲 李永平 朱海生 李大忠 |
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| 作者单位： | 福建省农业科学院蔬菜研究中心,福建福州 350013;福建省农业科学院作物研究所,福建福州 350013;福建省蔬菜工程技术研究中心,福建福州 350013 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD07B03);农业部农业公益性行业科研专项(nyhyzx07-007);福建省科技计划重大专项(2008NZ0002);福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y06);国家现代农业产业技术体系建设专项(CARS-25-G-20) |
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| 摘 要： | 以苦瓜杂交种“闽研2号”及其亲本为材料,利用RAPD标记技术对苦瓜杂交种的多态性进行分析.结果表明:在供试的200条RAPD引物中,筛选出1个引物S115能区分杂交种及其亲本种子.利用互补型RAPD引物S115对苦瓜杂交种“闽研2号”的纯度进行了快速鉴定,结果与田间鉴定结果一致.
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| 关 键 词： | 苦瓜 杂交种 纯度鉴定 RAPD |
RAPD Determination on Purity of Hybrid Bitter Gourd Seeds |
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| LIN Hui
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LI Yong-ping
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ZHU Hai-sheng
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LI Da-zhong.RAPD Determination on Purity of Hybrid Bitter Gourd Seeds[J].Fujian Journal of Agricultural Sciences,2011,26(6):977-980. |
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| Authors: | LIN Hui LI Yong-ping ZHU Hai-sheng LI Da-zhong |
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| Institution: | 1,2,3(1.Vegetable Centre,Fujian Academy of the Agricultural Science,Fuzhou,Fujian 350013,China; 2.Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China; 3.Engineering Research Center for Vegetable,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350013,China) |
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| Abstract: | Polymorphisms of the hybrid bitter gourd,"Minyan 2",and its parents lines were analyzed by using RAPD markers.Selected from 200 RAPD primers,the specific primer,S115,was found to be capable of differentiating the hybrid "Minyan 2" from its parents.Genetic purity of "Minyan 2" was further tested with S115 to agree with the field test results. |
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| Keywords: | Bitter gourd hybrid genetic purity RAPD |
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