| 不同培养基对秦党叶片及茎段培养的效应研究 |
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| 引用本文： | 张丽琼,周琼,柳林,屈国胜.不同培养基对秦党叶片及茎段培养的效应研究[J].湖北农业科学,2010,49(1). |
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| 作者姓名： | 张丽琼 周琼 柳林 屈国胜 |
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| 作者单位： | 安康学院农学与生命科学学院,陕西,安康,725000 |
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| 基金项目： | 安康学院等项科研计划资助项目 |
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| 摘 要： | 试验研究了不同培养基对秦党叶片及茎段培养的效应。结果表明,在光照强度1600 lx,温度25℃的条件下,利用组织培养技术快速繁殖秦党时,适宜的外植体为幼苗的茎段。茎段培养时,诱导愈伤组织并促进分化的适宜培养基组分为:MS+2,4-D 0.2 mg/L+蔗糖20 g/L+琼脂8 g/L。仅促进芽生长适宜的培养基组分为:MS+BA2.0 mg/L+IAA1.0 mg/L+蔗糖20 g/L+琼脂8 g/L。仅诱导愈伤组织适宜的培养基组分为:MS+BA1.0 mg/L+IAA1.0 mg/L+蔗糖20 g/L+琼脂8 g/L。
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| 关 键 词： | 培养基 秦党 叶片 茎段 培养 |
Effects of Different Culture Media on the Culture of Codonopsis tsinlingensis'Lamina and Stem |
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| ZHANG Li-qiong,ZHOU Qiong,LIU Lin,QU Guo-sheng.Effects of Different Culture Media on the Culture of Codonopsis tsinlingensis'Lamina and Stem[J].Hubei Agricultural Sciences,2010,49(1). |
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| Authors: | ZHANG Li-qiong ZHOU Qiong LIU Lin QU Guo-sheng |
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| Abstract: | It had studied the effect of different culture medium to the culture of Codonopsis tsinlingensis'lamina and stem,and the results showed that when the intensity of illumination was 1 600 lx and the temperature was 25℃,the suitable explant was the young stem when using tissue culture technology to breed Codonopsis tsinlingensis quickly.The culture medium that could inducement callus and accelerate differentiation was MS+2,4-D 0.2 mg/L+sucrose 20 g/L+agar 8 g/L.The culture medium that only could accelerate bud growth was MS+BA2.0 mg/L+IAA1.0 mg/L+sucrose 20 g/L+agar 8 g/L.The culture medium that only could inducement callus was MS+BA1.0 mg/L+IAA1.0 mg/L+sucrose 20 g/L+agar 8 g/L. |
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| Keywords: | culture medium Codonopsis tsinlingensis lamina stem culture |
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