| 中华倒刺鲃微卫星标记筛选及特征分析 |
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| 引用本文： | 熊美华,史 方,徐 念,阙延福,杨 钟,胡菊香,朱 滨.中华倒刺鲃微卫星标记筛选及特征分析[J].水生态学杂志,2011,32(1):40-44. |
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| 作者姓名： | 熊美华 史 方 徐 念 阙延福 杨 钟 胡菊香 朱 滨 |
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| 作者单位： | 水利部中国科学院水工程生态研究所,湖北,武汉,430079 |
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| 基金项目： | 乌江彭水水电站水生生物监测，乌江银盘水电站水生生物监测 |
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| 摘 要： | 以乌江流域中华倒刺鲃(Spinibarbus sinensis)为材料,构建了中华倒刺鲃微卫星富集文库。以乌江彭水电站所采集的32个个体的基因组DNA为模板,进行PCR扩增,结果筛选出8个多态性微卫星位点,其等位基因数4~10,多态信息含量0.6072~0.8515;观测杂合度0.5625~1.0000,期望杂合度0.6791~0.8795,Hardy中华倒刺鲃微卫星标记筛选及特征分析Weinberg平衡偏离指数-0.2009~0.3805;卡方检验结果表明,3个位点不同程度地偏离了Hardy中华倒刺鲃微卫星标记筛选及特征分析Weinberg平衡(P<0.05);各位点两两之间不存在显著的连锁不平衡现象。
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| 关 键 词： | 中华倒刺鲃 基因组富集文库 微卫星 多态性 |
| 收稿时间： | 2010/4/15 0:00:00 |
| 修稿时间： | 2010/11/29 0:00:00 |
Isolation and Characterization of Microsatellite Loci in Spinibarbus sinensis(Bleeker) |
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| xiong meim hu,shi fang,xu lian,que yan fu,yang zhong,hu ju xiang and zhu bing.Isolation and Characterization of Microsatellite Loci in Spinibarbus sinensis(Bleeker)[J].Journal of Hydroecology,2011,32(1):40-44. |
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| Authors: | xiong meim hu shi fang xu lian que yan fu yang zhong hu ju xiang and zhu bing |
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| Institution: | Institute of hydroecology, Ministry of Water Resources and Chinese Academy of Sciences |
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| Abstract: | The microsatellite markers of Spinibarbus sinensis were isolated in this study. A library of partial small size fractionated genomic DNA was constructed with the S. sinensis in Wujiang river. Eight microsatellite loci were isolated and characterized. These loci showed high levels of genetic polymorphism testing on 32 individuals sampled from Wujiang river. The number of alleles per locus ranged from 4 to 10. PIC changed from 0.6072 to 0.8515. The observed (Ho) and expected (He) heterozygosities were 0.5625 to 1.0000 and 0.6791 to 0.8795, respectively. Hardy-Weinberg departure value (D) ranged from -0.2009 to 0.3805. The Hardy-Weinberg Equilibrium (HWE) analysis showed that 3 loci deviated from genetic equilibrium significantly (P<0.05). No linkage disequilibrium was found. |
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| Keywords: | Spinibarbus sinensis enriched genomic library microsatellite polymorphism |
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