| 膜荚黄芪苯丙氨酸解氨酶特性的初步研究 |
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| 引用本文： | 孙晓丹,金美玉,严一字,全雪丽,吴松权.膜荚黄芪苯丙氨酸解氨酶特性的初步研究[J].辽宁林业科技,2010(5):1-2,35. |
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| 作者姓名： | 孙晓丹 金美玉 严一字 全雪丽 吴松权 |
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| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 该文初步研究了荚膜黄芪苯丙氨酸解氨酶(PAL)的基本特性。结果表明:PAL的最适反应时间为30min,PAL在Tris-HCl缓冲液中适宜的pH值为8.0,最适反应温度为37℃,PAL酶浓度过高或过低不利于酶的活力,PAL最适底物浓度为2mmol/L。
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| 关 键 词： | 膜荚黄芪 苯丙氨酸解氨酶 特性 |
Preliminary study on the characteristics of phenylalanine ammonia-lyase of Astragalus membranaceus |
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| SUN Xiao-dan,JIN Mei-yu,YAN Yi-zi,QUAN Xue-li,WU Song-quan.Preliminary study on the characteristics of phenylalanine ammonia-lyase of Astragalus membranaceus[J].Liaoning Forestry Science and Technology,2010(5):1-2,35. |
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| Authors: | SUN Xiao-dan JIN Mei-yu YAN Yi-zi QUAN Xue-li WU Song-quan |
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| Institution: | SUN Xiao-dan,JIN Mei-yu,YAN Yi-zi,QUAN Xue-li,WU Song-quan* (Agricultural College of Yanbian University,Longjing 133400,China) |
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| Abstract: | The basic characteristics of phenylalanine ammoma-lyase （PAL） of Astragalus membranaceus was studied. The results showed that the optimum reaction time was 30 min, the optimum pH in Tris-HCl buffer was 8.0, the optimum reaction temperature was 37 ℃, higher or lower concentration of PAL was not approprite for the PAL activity, and the optimum substrate concentration of PAL was 2 mmol/L. |
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| Keywords: | Astragalus membranaceus phenylalanine ammonia-lyase characteristics |
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