| 哲罗鱼几种同工酶表型的初步研究 |
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| 引用本文： | 马波,姜作发,尹家胜.哲罗鱼几种同工酶表型的初步研究[J].水产学杂志,2003,16(1):23-25. |
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| 作者姓名： | 马波 姜作发 尹家胜 |
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| 作者单位： | 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070 |
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| 摘 要： | 采用淀粉凝胶电泳方法，对乌苏里江下游虎头江段6尾哲罗鱼的肝脏、肌肉等组织的LDH、MDH、ME、IDH、SDH、EST等同工酶进行电泳，并对其表型进行生化遗传分析。LDH有3个位点，在各组织中的分布具显的差异，A与B亚基的结合受阻；SDH、ME和EST分别由1个、2个和4个基因位点编码；MDH和IDH各有3个位点。在检测到的6种同工酶共16个基因位点中未发现有多态位点，表明哲罗鱼在生化遗传上的变异比较贫乏。
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| 关 键 词： | 哲罗鱼 同工酶 淀粉凝胶电泳法 肝脏组织 肌肉组织 生化遗传分析 多态位点 生化遗传 鲑形目 |
| 文章编号： | 1005-3832(2003)01-0023-03 |
| 修稿时间： | 2003年3月1日 |
Primary Study of Iozyme Phenotypes of Huncho taimen Pallas |
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| MA Bo,JIANG Zuo-fa,YIN Jia-sheng.Primary Study of Iozyme Phenotypes of Huncho taimen Pallas[J].Chinese Journal of Fisheries,2003,16(1):23-25. |
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| Authors: | MA Bo JIANG Zuo-fa YIN Jia-sheng |
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| Abstract: | Six Huncho taimen samples were collected in Hutou section of Wusulijiang River. Starch gel electrophoresis were used to dectect expression of LDH, MDH, ME, IDH, SDH, EST isozymes in liver, muscle, eye and other tissues. Biochemical genetic analysis showed 6 isozymes were coded by 16 gene loci, none of which was polymorphic. Among those isozymes, LDH, MDH and IDH all contained 3 loci, particularly; the three loci of LDH distribution in different tissues exhibited significantly different, and its two subunits A and B were limited to associate. However, SDH, ME and EST consisted with 1, 2 and 4 loci, respectively. Compared with other species, H. taimen showed very low levels of genetic differentiation and genetic variabilit |
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| Keywords: | Huncho taimen Isozyme Electrophoresis |
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