| 食用仙人掌的离体培养及其快速繁殖 |
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| 引用本文： | 陈丽静,潘英,马慧,刘少霞,姜明兰,钟文田.食用仙人掌的离体培养及其快速繁殖[J].园艺学报,2001,28(4):327-330. |
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| 作者姓名： | 陈丽静 潘英 马慧 刘少霞 姜明兰 钟文田 |
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| 作者单位： | ( 沈阳农业大学生物科学技术学院, 沈阳110161 ; 沈阳农业大学生物科学技术学院海城天成产学研基地, 海城114200) |
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| 摘 要： | 研究了食用仙人掌离体快繁的若干影响因素。适宜的培养基为: 不定芽诱导, MS + 6-BA 3. 0 mg/L + NAA 0. 1 mg/L ; 继代和增殖, MS + 6-BA 1 mg/L + KT 0. 5 mg/L + NAA0. 3 mg/L , 继代培养30 d , 平均增殖倍数为8 ; 生根培养基为1/ 2 MS + NAA 0. 1 mg/L + IBA 0. 1 mg/L。在培养过程中, 有些外植体膨大, 并有疏松的淡黄色颗粒状愈伤组织出现, 从愈伤组织表面分化出芽; 有些外植体不膨大, 无愈伤组织出现, 直接分化出丛生芽。过渡移栽基质为田园土∶珍珠岩∶河沙= 1∶1∶1 , 试管生根苗根长约1 cm时移栽, 成活率最高可达95 %以上。
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| 关 键 词： | 仙人掌 食用仙人掌 植物组织培养 快速繁殖 |
| 文章编号： | 0513-353X(2001)04-0327-04 |
| 修稿时间： | 2000年11月1日 |
Tissue Culture and High Frequency Propagation of Opuntia milpa alta Haw |
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| Chen Lijing,Pan Ying,Ma Hui,Liu Shaoxia,Jiang Minglan,and Zhong Wentian.Tissue Culture and High Frequency Propagation of Opuntia milpa alta Haw[J].Acta Horticulturae Sinica,2001,28(4):327-330. |
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| Authors: | Chen Lijing Pan Ying Ma Hui Liu Shaoxia Jiang Minglan and Zhong Wentian |
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| Institution: | ( Biotechnology Institute of Shenyang Agricultural University , Shenyang 110161) |
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| Abstract: | Effects of several factors such as medium,cutting materials and plant growth regulators on in vitro culture and rapid propagation of Opuntia milpa alta Haw.were investigated.Using the Opuntia milpa alta Haw.stem as explant,a method for high frequency propagation of Opuntia milpa alta Haw.was established.The results of the experiments suggested that the appropriate me dium for different culture stages were:(1)Germination of adventitious buds:MS 6 BA 3.0mg/L NAA 0.1mg/L. On this medium shoot multiplication occurred at a 8 fold rate after 30 days;(2)Differentiation and subculture of rosette buds:MS 6 BA 1mg/L KT 0.5mg/L NAA 0.3mg/L;(3)Rootage:1/2 MS NAA 0.1mg/L IBA 0.1mg/L.The shoots could be directly rooted in transplantation medium(soil,vermiculite and sand in equal proportion)with up to 95% survival. |
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| Keywords: | Opuntia Mill Opuntia milpa alta Haw Plant tissue culture High frequency propagation |
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