| 特种水生蔬菜刺苦草初代培养研究初探 |
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| 作者姓名： | 陈 寒 徐文娟 李 勇 赵 迪 |
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| 作者单位： | 安徽农业大学园艺学院，合肥 230036 |
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| 基金项目： | 安徽省科技攻关项目，安徽省教育厅科研项目 |
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| 摘 要： | 选取优质的刺苦草的根状茎、芽、叶为外植体,以MS为基本培养基适时添加适量的生长素和细胞分裂素进行离体培养.结果表明:以芽为外植体,1%升汞消毒8min,以MS为培养基,添加0.2～2mg/LBA、0.2～2mg/LNAA、0.2～2mg/LIBA、蔗糖30g/L、琼脂10g/L,BA/NAA为3:1和1:1时和BA/IBA为1:1和1:10时,植株生长健壮,叶片颜色青翠,幼茎生长得又快又高.
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| 关 键 词： | 刺苦草 组织培养 快速繁殖 |
Research on the Primary Culture of the Special Aquatic Vegetable Vallisneria Spinulosa Yan |
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| Authors: | Chen Han Xu Wenjuan Li Yong Zhao Di |
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| Institution: | Horticultural College of Anhui Agricultural University, Hefei 230036 |
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| Abstract: | The shoot tips of Vallisneria Spinulosa Yan were used as explants in the test of tissue culture and rapid propagation, the direct method of differentiation of young sprout by inducing embryoid was used with the basic MS culture medium with the different sorts and concentrations of phytohormone. The results showed that embryoid was induced and the young sprout formed from the plumules with the basic MS culture medium with 0.2-2mg/LBA, 0.2-2mg/LNAA, 0.2-2mg/LIBA, 30g/Lsugar, 10g/Lagar. When BA/NAA=3:1 and 1:1 or BA/IBA=1:1 and 1:10, the plants were getting the seeding that grew orderly and vigorously with fresh green leaves and higher leaf-like stems. |
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| Keywords: | Vallisneria Spinulosa Yanzz tissue culturezz rapid propagationzz |
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