| 三种方法对肉松中胆固醇含量的测定及比较 |
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| 引用本文： | 朱炳祺,刘柱,陈万勤,罗金文.三种方法对肉松中胆固醇含量的测定及比较[J].湖北农业科学,2017,56(5). |
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| 作者姓名： | 朱炳祺 刘柱 陈万勤 罗金文 |
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| 作者单位： | 浙江省食品药品检验研究院,杭州,310000 |
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| 基金项目： | 浙江省食品药品检验研究院科研项目 |
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| 摘 要： | 采用直接皂化方法对样品进行前处理,用乙醚和石油醚混合液(1∶1)萃取,用气相色谱、液相色谱和比色法分别进行检测。气相、液相色谱法在25~500 mg/L范围内具良好的线性关系(R20.999),比色法在0~42 mg/L范围内具良好的线性关系(R20.997)。液相、气相色谱和比色法3个加标浓度平均回收率分别为105.5%、100.1%和102.3%,检出限分别为0.1、0.4和2.1 mg/100 g,对肉松中胆固醇含量测得值分别为52.2、51.2和102 mg/100 g。3种方法均很好地满足方法学要求,其中比色法体现总固醇类化合物的含量;气相色谱法和液相色谱法专属性更强、灵敏度更高,能更准确地反应样品中胆固醇含量。
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| 关 键 词： | 胆固醇 肉松 气相色谱法 液相色谱法 比色法 |
Determination of Cholesterol Contents in Dried Meat Floss and Comparison of 3 Different Methods |
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| ZHU Bing-qi,LIU Zhu,CHEN Wan-qin,LUO Jin-wen.Determination of Cholesterol Contents in Dried Meat Floss and Comparison of 3 Different Methods[J].Hubei Agricultural Sciences,2017,56(5). |
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| Authors: | ZHU Bing-qi LIU Zhu CHEN Wan-qin LUO Jin-wen |
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| Abstract: | Saponification was applied in sample pretreatment, and cholesterol was extracted by a mixed solvent of diethyl ether and petroleum ether (1:1,V/V),then analyzed by liquid chromatography(LC),gas chromatography(GC) and colorimetric method respectively. The results showed that good linearity were obtained in the range of 25~500 mg/L by using chromatogra-phy (R2>0.999) and in 0~42 mg/L by colorimetric method (R2>0.997). By using LC,GC and colorimetric method,the average recovery were 105.5%,100.1% and 102.3%,the limit of detection (LOD) were 0.1 mg/100 g,0.4 mg/100 g and 2.1 mg/100 g, and the cholesterol content in dried meat floss were 52.2 mg/100 g, 51.2 mg/100 g and 102 mg/100 g respectively. All three analytical methods were validated, among which the colorimetric method revealed the total steroid content,and LC,GC were more accurate and sensitive in cholesterol analysis than colorimetric method. |
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| Keywords: | cholesterol dried meat floss gas chromatography liquid chromatography colorimetric method |
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