| 紫外分光光度法快速测定龙泉破壁灵芝孢子粉三萜含量 |
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| 引用本文： | 叶银鹏,叶伟平,周虎,饶璐珊.紫外分光光度法快速测定龙泉破壁灵芝孢子粉三萜含量[J].湖北农业科学,2017,56(2). |
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| 作者姓名： | 叶银鹏 叶伟平 周虎 饶璐珊 |
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| 作者单位： | 1. 龙泉市产品质量监督检验所,浙江 龙泉,323700;2. 龙泉市农业局,浙江 龙泉,323700 |
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| 摘 要： | 选取5种龙泉破壁灵芝孢子粉为研究对象,使用紫外分光光度法,建立了一种快速、简单、高灵敏度,专门针对灵芝孢子粉中三萜测定的方法。结果表明,该方法测得龙泉破壁灵芝孢子粉三萜含量为2.94%~3.43%。熊果酸浓度在20~140μg范围内呈良好的线性关系,精密度(RSD)为0.88%,回收率为88.6%~92.5%,完全能够满足于破壁灵芝孢子粉中三萜成分的分析研究。
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| 关 键 词： | 紫外分光光度法 灵芝孢子粉 三萜 龙泉市 |
Rapib Determination of Triterpene in Broken Cellular Wall of Ganoderma luCidum Spore in Longquan by Ultraviolet Spectrometry |
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| YE Yin-peng,YE Wei-ping,ZHOU Hu,RAO Lu-shan.Rapib Determination of Triterpene in Broken Cellular Wall of Ganoderma luCidum Spore in Longquan by Ultraviolet Spectrometry[J].Hubei Agricultural Sciences,2017,56(2). |
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| Authors: | YE Yin-peng YE Wei-ping ZHOU Hu RAO Lu-shan |
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| Abstract: | 5 kinds of broken cellular wall of ganoderma lucidum spore in Longquan were chosen as study subjects. An analyt-ical method was developed to determine triterpene in ganoderma lucidum spore by ultraviolet spectrometry. This method was rapid,simple and sensitive. The results indicated that the standard curves of ursolic acid showed good linearity in the range ration of 20~140μg,and the method precision (RSD) was 0.88%. The recoveries of triterpene ranged from 88.6% to 92.5%. The proposed method can be used for the determination of triterpene in broken cellular wall of ganoderma lucidum spore. |
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| Keywords: | ultraviolet spectrometry ganoderma lucidum spore powder triterpene Longquan city |
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