| 考马斯亮蓝法对烟草薄片涂布液中蛋白质含量的测定 |
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| 引用本文： | 杨静,白冰,王宁,张改红.考马斯亮蓝法对烟草薄片涂布液中蛋白质含量的测定[J].湖北农业科学,2017,56(5). |
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| 作者姓名： | 杨静 白冰 王宁 张改红 |
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| 作者单位： | 1. 郑州轻工业学院食品与生物工程学院,郑州,450000;2. 河南卷烟工业烟草薄片有限公司,河南 许昌,461000 |
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| 基金项目： | 郑州轻工业学院级校科研基金项目，河南省科技攻关项目 |
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| 摘 要： | 采用考马斯亮蓝法对烟草薄片涂布液中的蛋白质含量进行了测定,建立了快速、灵敏的蛋白质检测方法。结果表明,蛋白质含量在14~140μg/m L与吸光度呈良好的线性关系(R2=0.999 5),样品平均回收率为101.5%,RSD为1.4%。该方法简便、快速、准确度高、重现性好,可以作为烟草薄片涂布液及烟草料液中蛋白质测定的有效方法。
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| 关 键 词： | 薄片涂布液 蛋白质 考马斯亮蓝法 |
Determination of Protein Content in Reconstituted Tobacco Coating Liquid by Coomassie Brilliant Blue Method |
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| YANG Jing,BAI Bing,WANG Ning,ZHANG Gai-hong.Determination of Protein Content in Reconstituted Tobacco Coating Liquid by Coomassie Brilliant Blue Method[J].Hubei Agricultural Sciences,2017,56(5). |
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| Authors: | YANG Jing BAI Bing WANG Ning ZHANG Gai-hong |
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| Abstract: | A rapid and sensitive Coomassie brilliant blue method was developed for protein determination in reconstituted tobacco coating liquid. The results showed that,the protein content had a good linear relationship with the absorbance in the range of 14~140μg/mL (R2=0.9995). The average recovery was 101.5% and RSD was 1.4%. The method was simple,rapid,accurate and have high reproducibility,which could be used to determine the protein content in reconstituted tobacco coating liquid and tobacco sauce as an efficient method. |
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| Keywords: | reconstituted tobacco coating liquid protein coomassie brilliant blue method |
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