| 河北省4个黑猪群体的遗传多样性分析 |
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| 引用本文： | 赵思思,贾青,胡慧艳,李赛,张秋良,姚恒江.河北省4个黑猪群体的遗传多样性分析[J].河北农业大学学报,2017,40(2). |
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| 作者姓名： | 赵思思 贾青 胡慧艳 李赛 张秋良 姚恒江 |
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| 作者单位： | 1. 河北农业大学 动物科技学院,河北 保定,071000;2. 河北农业大学 动物科技学院,河北 保定 071000;国家北方山区农业工程技术研究中心,河北 保定 071000;河北省山区农业工程技术研究中心,河北 保定 071000;3. 河北省辛集市正农牧业有限公司,河北 辛集,052371;4. 河北省畜牧兽医研究所,河北 保定,071000;5. 易县畜牧站,河北 易县,074200 |
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| 摘 要： | 为合理利用地方猪遗传资源,随机选取河北省4个市的4个黑猪群体,采用FAO-ISAG联合推荐的8个微卫星标记对各样本群体的等位基因数、有效等位基因数、杂合度、多态信息含量及F-统计量进行检测。结果显示,8个微卫星座位共检测到66个等位基因。有效等位基因数(3.103~5.349),观察杂合度(0.346~0.437),期望杂合度(0.638~0.790)和多态信息含量(0.578~0.762)4个指标,表明4个猪群体具有丰富的多样性。所检测的微卫星位点均偏离平衡状态,主要是由于起始群体规模小或公猪家系少所致。F统计量结果表明,4个猪群体都存在一定程度的近交。依据对4个猪群的分析结果,群体1、2可通过扩大血统数,以减缓近交程度;群体3、4应开展选育,以提高群体一致性。
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| 关 键 词： | 地方猪 遗传多样性 微卫星 |
Genetic diversity of four native pig populations in Hebei province |
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| Abstract: | In order to reasonably utilize the local pig genetic resources,4 black pig populations in different cities of Hebei Province were randomly selected,and the allele numbers,effective number of alleles,heterozygosity,polymorphism information content and F-statistic of sample populations were detected by 8 microsatellite markers recommended by Food and Agriculture Organization and International Society for Animal Genetics(FAO-ISAG).The result showed that 66 alleles were observed at 8 loci.The genetic diversity were rich in all the 4 populations indicated by the 4 indices:effective number of alleles(3.103～5.349),observed heterozygosity (0.346～0.437),expected heterozygosity(0.638～0.790)and polymorphism information content(0.578～0.762).The main reasons that all the 8 tested loci deviated from the equilibrium were small population size and limited boar families.The F-statistic results implied that there was certain inbreeding in the populations.Based on the results and analysis above,it is suggested that group1 and group 2 may increase quantity of bloodline to slow down the inbreeding status,and group 3 and group 4 should conduct selected breeding works to improve the population consistency. |
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| Keywords: | local pig population genetic diversity microsatellite |
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