Platelet activating factor as a mediator of equine cell locomotion |
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| Authors: | J Dawson P Lees A D Sedgwick |
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| Institution: | (1) R.V.C. Inflammation Group, The Royal Veterinary College, University of London, Hawkshead Campus, North Mymms, AL9 7TA Hatfield, Hertfordshire, Great Britain |
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| Abstract: | Equine polymorphonuclear (PMN) and mononuclear (MN) leucocytes were separated on Percoll gradients and used to study the chemoattractant properties of the polar ether-linked phospholipid, platelet activating factor (PAF). Six concentrations of PAF ranging from 1 ng/ml to 100  g/ml were studied in each of two in vitro assay systems, the agarose microdroplet and a microfilter technique. Very significant (p<0.01) increases in the movement of both PMN and MN cells were obtained with most concentrations of PAF. In two instances there was no apparent concentration-response relationship, although the action of PAF was approximately bell-shaped in two others. The possible significance of these findings for equine inflammatory conditions is discussed. |
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