| 鸡传染性贫血病毒VP2基因的克隆和原核表达 |
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| 引用本文： | 赵锋,李学花,林世武,徐增强,李铁全,邱建东,李进,樊靖.鸡传染性贫血病毒VP2基因的克隆和原核表达[J].中国动物检疫,2009,26(11):34-36. |
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| 作者姓名： | 赵锋 李学花 林世武 徐增强 李铁全 邱建东 李进 樊靖 |
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| 作者单位： | 太原市动物卫生监督所,山西太原,030027 |
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| 摘 要： | 从组织病料中提取到的鸡贫血病毒核酸经PCR扩增得到vp2基因,并将其克隆到表达载体pET32a上,重组菌经IPTG诱导,SDS-PAGE分析,结果VP2基因在大肠杆菌中成功表达。以表达产物免疫小鼠4次,制备了抗CAVVP2的多克隆抗体。通过对CAV感染的MSB1细胞做间接免疫荧光试验(IFA),结果为阳性。这表明表达产物保留了CAV相关的抗原性。为进一步研究CIA临床诊断奠定了基础。
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| 关 键 词： | 鸡贫血病毒 VP2 基因表达 多抗血清 间接免疫荧光试验(IFA) |
Cloning and prokaryotic expression of chicken anemia virus VP2 gone |
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| Zhao Feng,Li Xuehu,Lin Shiwu,Xu Zengqiang,Li Tiequan,Qiu Jiandong,Li Jin,Fan Jing.Cloning and prokaryotic expression of chicken anemia virus VP2 gone[J].China Journal Of Animal Quarantine,2009,26(11):34-36. |
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| Authors: | Zhao Feng Li Xuehu Lin Shiwu Xu Zengqiang Li Tiequan Qiu Jiandong Li Jin Fan Jing |
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| Institution: | (Taiyuan Municipal Animal Health Supervision Institute, Shanxi 030027) |
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| Abstract: | Chicken anemia virus genome extracted from liver tissue, was amplified with PCR and VP2 gene was gained,then inserted into pET32a vector and transformed into competent E. coli cell plys. The recombinant bacteria was induced by IPTG and analyzed with SDS-PAGE. The result showed that gene encoding VP2 was expressed in E. coli at high level. The anti-CAV polyclonal antibody was produced in mice immunized four times with the expressed protein ,and it showed positive reaction with CAV-infected MSB1 cells in indirect immunofluorescent assay (IFA). This result showed that the expressed protein retained the CAV-related antigenicity can will be used to the development of clinical diagnosis of CIA. |
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| Keywords: | VP2 |
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