| 胎猪胰腺干细胞的分离与鉴定 |
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| 引用本文： | 王赟,赵婷,乔海,杨春荣,张翊华,王华岩,窦忠英.胎猪胰腺干细胞的分离与鉴定[J].农业生物技术学报,2007,15(4):622-627. |
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| 作者姓名： | 王赟 赵婷 乔海 杨春荣 张翊华 王华岩 窦忠英 |
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| 作者单位： | 西北农林科技大学国家干细胞生物技术中心陕西分中心,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划);教育部科学技术研究项目;国家自然科学基金;陕西省重大科技专项计划 |
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| 摘 要： | 从胎猪胰腺分离得到一株细胞，该细胞经免疫组化检测表达PDX-1，GLUT-2，PCNA，Glucagon，somatostatin，polypeptide，弱表达nestin，目前细胞已传至58代。其在体外的生长行为有类似间充质干细胞样生长形态，经诱导后可分化为具有三维立体结构的类胰岛细胞团。成团后细胞强表达insulin、ck-19等标志，放免测定显示诱导成团后细胞胰岛素分泌量增加，显著高于未诱导细胞（P<0.05）。说明胰腺内存在着多潜能干细胞，本方法分离到胎猪胰腺干细胞，经诱导分化可形成有功能的胰岛样细胞团并释放胰岛素,有望为进一步研究异种胰腺干细胞移植治疗糖尿病提供材料。
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| 关 键 词： | 胎猪 胰岛 胰腺干细胞 糖尿病 |
| 文章编号： | 1006-1304（2007）04-0622-06 |
| 收稿时间： | 2006-12-31 |
| 修稿时间： | 2007-03-08 |
Isolation and Identification of Porcine Fetus Pancreatic Stem Cells |
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| WANG Yun,ZHAO Ting,QIAO Hai,YANG Chun-rong,ZHANG Yi-hua,WANG Hua-yan,DOU Zhong-ying.Isolation and Identification of Porcine Fetus Pancreatic Stem Cells[J].Journal of Agricultural Biotechnology,2007,15(4):622-627. |
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| Authors: | WANG Yun ZHAO Ting QIAO Hai YANG Chun-rong ZHANG Yi-hua WANG Hua-yan DOU Zhong-ying |
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| Abstract: | Abstruct：Cells derived from porcin fetus expresse PDX-1, GLUT-2,PCNA, Glucagon, somatostatin，polypeptide.Nestin was weakly expressed.These cells have strong viability,It were subcloned over 58 passages during 13 months and identified as pancreatic stem cells by immunochemical method。
The cell can form three-diemention islet-like structure by induction in vitro.It stongly express insulin and ck-19 after forming ICCs.This result reveal that the cell is pancreatic stem cells and pancreatic stem cells do exist in pancreas,It maintains the steady of pancreas by neogenesis and differation.
At present,our laboratory can isolate and expand this cell steadily.This study can provide reference for isolation and expansion of pancreatic stem cells.Also,it can provide new source for diabetes therapy. |
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| Keywords: | porcine fetus islet pancreatic stem cells diabetes mellitus |
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