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| 猪副粘病毒间接ELISA方法的建立 | |
| 引用本文： | 孙凤萍,胡建华,高骏,吴祖立,李春华,邵伟娟,谢建云,高诚.猪副粘病毒间接ELISA方法的建立[J].上海农业学报,2007,23(4):34-37. |
| 作者姓名： | 孙凤萍 胡建华 高骏 吴祖立 李春华 邵伟娟 谢建云 高诚 |
| 作者单位： | 1. 上海市农业科学院畜牧兽医研究所,上海,201106 2. 上海市实验动物质量监督检验站,上海,200032 3. 上海交通大学农业与生物学院,上海,201101 |
| 摘 要： | 以临床分离的猪副粘病毒作为抗原,通过SPF鸡胚进行病毒培养和增殖,再经过差速离心纯化后作为ELISA包被抗原,建立了猪副粘病毒全病毒间接ELISA诊断方法。试验表明:此方法具有敏感性高、特异性好的优点,为快速诊断新型猪传染病——猪副粘病毒(PPMV)感染提供了便利的途径。 |
| 关 键 词： | 猪副粘病毒 间接ELISA量 |
| 文章编号： | 1000-3924（2007）04-34-04 |
| 收稿时间： | 2006-10-06 |
| 修稿时间： | 2007-09-27 |
Establishment of indirect ELISA for porcine paramyxovirus |
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| SUN feng-ping,HU Jian-hua,GAO Jun,WU Zu-li,LI Chun-hua,SHAO Wei-juan,XIE Jian-yun,GAO Cheng.Establishment of indirect ELISA for porcine paramyxovirus[J].Acta Agriculturae Shanghai,2007,23(4):34-37. | |
| Authors: | SUN feng-ping HU Jian-hua GAO Jun WU Zu-li LI Chun-hua SHAO Wei-juan XIE Jian-yun GAO Cheng |
| Abstract: | Porcine paramyxovirus(PPMV)isolated from some pig farms in Shanghai and Zhejiang was used as a antigen and SPF chick embryos were used to proliferate the virus.By the differential centrifugation method the virus was purified and used as ELISA coating antigen.The indirect ELISA for porcine paramyxovirus was established.The comparative tests showed that this method has high sensitivity and good specificity and offers a convenient way to detect the PPMV infection. |
| Keywords: | Porcine paramyxovirus Indirect ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |