| 禽白血病病毒抗体间接ELISA 检测方法的建立及初步应用 |
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| 引用本文： | 毛娅卿.禽白血病病毒抗体间接ELISA 检测方法的建立及初步应用[J].中国兽药杂志,2013,47(11):5-9. |
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| 作者姓名： | 毛娅卿 |
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| 作者单位： | 中国兽医药品监察所 |
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| 摘 要： | 以大肠杆菌表达、纯化的禽白血病病毒（ALV）重组P27蛋白为包被抗原,建立了检测ALV-P27抗体的间接ELISA方法,为禽白血病流行病学调查提供了一种简便的血清学检测方法.该方法与9种禽常见传染病病毒阳性血清及大肠杆菌阳性血清无交叉反应,具有较好的特异性;批内、批间重复试验变异系数均小于10％,具有良好的重复性;新建立的ELISA方法比IDEXX公司生产的ALVA、B亚群抗体检测试剂盒和J亚群抗体检测试剂盒相对于免疫荧光试验（IFA）有更高的符合率.
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| 关 键 词： | 禽白血病病毒 重组P27蛋白 间接ELISA |
| 收稿时间： | 7/3/2013 12:00:00 AM |
| 修稿时间： | 2013/9/25 0:00:00 |
Development and Preliminary Application of an Indirect-ELISA Diagnostic Method for Detection of Avian Leukosis Virus Antibodies |
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| Institution: | China Institute of Veterinary Drug Control,Beijing,100081 |
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| Abstract: | An indirect -enzyme -linked immunosorbent assay （iELISA） method was developed for detecting antibodies against Avian leukosis virus （ALV） using recombinant P27 protein. There were no cross reaction with antiserum against 9 kinds of avian infectious disease agent and antiserum against E. coll. Variation coefficients were less than 10% in the intro - batch and in inter - batch tests. The protocol of iELISA was optimized and the method showed more sensitive and specificity than the commercial IDEXX ALV antibody test kit when immunoflourescentassay （IFA） was used as a reference. The results showed that the developed iELISA was specific and can potentially be used for detection of anti - ALV antibodies on a large scale. The indirect ELISA assay would provide a simple and rapid means of monitoring ALV infection. |
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| Keywords: | avian leukosis virus recombinant P27 protein indirect - ELISA |
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