| 黄竹细胞悬浮培养和原生质体分离* |
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| 引用本文： | 阙国宁,诸葛强.黄竹细胞悬浮培养和原生质体分离*[J].林业科学研究,1994,7(1):44-47. |
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| 作者姓名： | 阙国宁 诸葛强 |
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| 作者单位： | 中国林业科学研究院亚热带林业研究所 |
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| 摘 要： | 以牡竹属黄竹（ＤｅｎｄｒｏｃａｌａｍｕｓｍｅｍｂｒａｎｃｅｕｓＭｕｎｒｏ）的竹节及无菌苗根颈为外植体，培养于含有５ｍｇ／Ｌ２，４－Ｄ、０．２ｍｇ／ＬＫＴ和２００ｍｇ／ＬＬＨ的ＭＳ培养基上诱导愈伤组织，然后移至相同成份或不同浓度２，４－Ｄ的液体培养基中，进行悬浮培养，以建立分散性良好的悬浮细胞系。利用悬浮细胞和无菌苗叶片经酶解获大量原生质体，其产量分别约为每克鲜重２．５×１０５个和５×１０５个，活力均可达８０％。
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| 关 键 词： | 黄竹 细胞悬浮培养 原生质体分离 |
| 收稿时间： | 1993/8/10 0:00:00 |
Study on Cell Suspension Culture and lsolation of Protoplfast Of Dendrocalamus membranceus |
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| Que Guoning and Zhuge Qiang.Study on Cell Suspension Culture and lsolation of Protoplfast Of Dendrocalamus membranceus[J].Forest Research,1994,7(1):44-47. |
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| Authors: | Que Guoning and Zhuge Qiang |
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| Institution: | The Research Institute of Subtropical Forestry, CAF Fuyang, Zhejiang 311400;The Research Institute of Subtropical Forestry, CAF Fuyang, Zhejiang 311400 |
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| Abstract: | Root crowns of plantlets and young stems with joint of Dendrocalamus membranceus Munro were cultured on MS agar medium supplemented with 5 mg/L 2, 4-D,0.2mg/L KT and 200 mg/L LH, calli in rapid growth were formed. Then, the calli were transferred to a liquid medium with the same medium composition or with diverse levels of 2, 4-D, the cell suspension culture which consisted maily of cell or cell aqqregates, was established. A great amount of protoplasts were released from mesophyll of sterile seedlings and cell suspension cultures, with protoplast yields 5×105/g FW and 2.5×105/g FW respectively,and viability above 80%. |
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| Keywords: | Dendrocalamus membranceus cell suspension culture protoplast isolation |
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