Comparison of fertility on intrauterine insemination between cryopreserved ejaculated and cauda epididymal sperm in dogs |
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Authors: | Hori Tatsuya Matsuda Yasuyoshi Kobayashi Masanori Kawakami Eiichi Tsutsui Toshihiko |
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Institution: | Department of Reproduction, Nippon Veterinary and Life Science University, Musashino-shi, Tokyo 180–8602, Japan. t-hori@nvlu.ac.jp |
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Abstract: | The fertility was compared between ejaculated and cauda epididymal sperm sensitized with prostatic fluid in dog after freeze-thawing using the fertility of ova from the contralateral ovary after injection (2 × 10(8) sperm) into dog uterus on the unilateral ovariectomized side, on the basis of the presence or absence of conception. No significant difference was observed in sperm quality after freeze-thawing between the two groups and conception rates were equivalent and low. Therefore, to achieve a high fertility by intrauterine insemination of canine frozen-thawed ejaculated and cauda epididymal sperm, intrauterine insemination on both sides is recommended, rather than insemination with a lot of sperm of the uterine horn on one side. |
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