| HPLC法测定不同产地女贞叶中熊果酸的含量 |
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| 引用本文： | 王丽,闫春生,贾安,信建豪,黄涛.HPLC法测定不同产地女贞叶中熊果酸的含量[J].安徽农业科学,2014(28):9738-9739. |
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| 作者姓名： | 王丽 闫春生 贾安 信建豪 黄涛 |
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| 作者单位： | 黄河科技学院,河南郑州,450006 |
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| 摘 要： | 目的]用高效液相色谱法（HPLC）考察5个不同产地女贞叶中熊果酸的含量.方法]用高效液相色谱法所用色谱柱为十八烷基硅烷键合硅胶色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-甲醇-0.5％醋酸铵溶液（67∶12∶21）,检测波长为210 nm.结果]方法学考察结果,回归方程为Y=4.715 5X＋ 17.918,R2 =0.999 4,熊果酸含量在5.21～125.12 μg/ml浓度范围内线性关系良好.用该方法测得5个不同产地女贞叶中熊果酸的含量存在差异,其中上海市产的含量最高,为2.157 4 mg/g,南阳唐河产的含量最低,为0.863 0mg/g.结论]同一采集时间,不同产地的女贞叶因环境因素,所含熊果酸的含量差异较大.
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| 关 键 词： | 女贞叶 熊果酸 HPLC 含量测定 |
Determination of the Content of Ursolic Acid in Folium Ligustrum lucidus from Different Habitats by HPLC |
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| Institution: | WANG Li, YAN Chun-sheng, JIA An et al (Huanghe Science and Tecimology College, Zhengzhou, Henan 450006) |
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| Abstract: | Objective]The content of ursolic acid in folium Ligustrum lucidus from different habitats was investigated by HPLC.Method]Chromatographic column is eighteen alkyl silane bonded silica gel column chromatography （4.6 mm × 250 mm,5 μm）,the mobile phase is acetonitrile-methanol-0.5 ％ ammonium acetate （67∶12∶21）,the detection wavelength is 210 nm.Result]Methodological study results：the regression equation：Y =4.715 5X ＋ 17.918,R2 =0.999 4,the content of ursolic acid in 5.21-125.12 μg/ml,the concentration of the linear relationship is good.The content of ursolic acid in folium Ligustrum lucidus from different habitats existis differences,the highest content of Shanghai produced is up to 2.157 4 mg/g,the lowest content produced in Nanyang Tanghe is 0.863 0 mg/g.Conclusion]At the same acquisition time,the content of ursolic acid in folim Ligustrum lucidus from different habitats has significant differences because of environmental factors. |
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| Keywords: | Ligustrum lucidus Ursolic acid HPLC Content determination |
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