| 利用抑制差减杂交技术分离棕色棉纤维特异表达基因 |
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| 引用本文： | 李艳军,张新宇,杨会娜,田新惠,孙杰.利用抑制差减杂交技术分离棕色棉纤维特异表达基因[J].分子植物育种,2009,7(1). |
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| 作者姓名： | 李艳军 张新宇 杨会娜 田新惠 孙杰 |
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| 作者单位： | 石河子大学农学院,新疆兵团绿洲生态农业重点实验室,石河子,832003 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，兵团科技局基础研究资金项目，石河子大学高层次人才启动资金项目 |
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| 摘 要： | 采用棕色棉新彩棉5号为Tester,其白色近等基因系NIL-C5为Driver,通过抑制差减杂交法(suppressive subtractive hybridization,SSH)构建了一个棕色棉纤维色素差异表达基因差减文库.从文库中随机挑选400个克隆,将这些克隆的PCR产物点膜进行反向Northern杂交检测,挑选20个差异明显的克隆进行序列分析,结果表明:有5个cDNA片段与已知的功能基因序列相似,其中1个cDNA片段与合成色素的重要酶基因4-香豆酸:CoA连接酶(4CL)基因有较高的同源性,其它cDNA片段在数据库中未找到同源序列.
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| 关 键 词： | 彩棉 抑制扣除杂交 差异基因 |
Isolation of Specifically Expressed Genes in Brown Colored Cotton by Suppression Subtractive Hybridization |
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| Li Yanjun,Zhang Xinyu,Yang Huina,Tian Xinhui,Sun Jie.Isolation of Specifically Expressed Genes in Brown Colored Cotton by Suppression Subtractive Hybridization[J].Molecular Plant Breeding,2009,7(1). |
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| Authors: | Li Yanjun Zhang Xinyu Yang Huina Tian Xinhui Sun Jie |
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| Institution: | Li Yanjun Zhang Xinyu Yang Huina Tian Xinhui Sun Jie College of Agriculture,Key Laboratory of Oasis Ecology Agriculture of Bingtuan,Shihezi University,Shihezi,832003 |
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| Abstract: | A subtracted cDNA library was constructed via suppressive subtractive hybridization (SSH), in which the brown colored cotton Xincaimian 5 was utilized as tester while driver is its white colored susceptible near-iso-genic line NIL-C5. Total 400 clones were identified from screening of the subtracted library by PCR, and further verified by Reverse Northern Dot-Blot. 20 differentially expressed clones were selected for sequencing analyses, of which the results were aligned with GenBank databases via BLASTX pr... |
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| Keywords: | Colored cotton Suppression subtractive hybridization Differentially expressed gene |
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