| 栽培种花生株型相关性状的QTL定位 |
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| 引用本文： | 孟鑫浩,邓洪涛,李丽,崔顺立,Charles Y.Chen,侯名语,杨鑫雷,刘立峰.栽培种花生株型相关性状的QTL定位[J].中国农业科学,2021,54(8):1599-1612. |
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| 作者姓名： | 孟鑫浩 邓洪涛 李丽 崔顺立 Charles Y.Chen 侯名语 杨鑫雷 刘立峰 |
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| 作者单位： | 1河北农业大学农学院/华北作物改良与调控国家重点实验室/河北省种质资源实验室,中国河北保定 0710012河北工程大学园林与生态工程学院,中国河北邯郸 0560383奥本大学/作物、土壤与环境科学系,美国奥本 36849 |
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| 基金项目： | 国家现代农业产业技术体系建设项目(CARS-13);国家自然科学基金(31701459,31771833);河北省科技计划(16226301D);河北省现代农业产业技术体系油料创新团队项目(HBCT2018090202);河北省青年拔尖人才资助项目(0602015);河北农业大学大学生创新创业训练计划(2018138) |
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| 摘 要： | 【目的】栽培种花生是世界范围内重要的油料作物和经济作物,其株型相关性状是典型的数量性状,亦是重要的农艺性状,与产量和机械化收获密切相关。对花生株型相关性状进行遗传分析和QTL定位,筛选与之紧密连锁的分子标记,有助于花生的品种保护和品种鉴别,为花生株型分子育种提供重要的理论依据。【方法】以直立型花生品种冀花5号和匍匐型M130为亲本构建的包含321个家系的RIL群体为研究材料,于2016—2018年分别在海南市、邯郸市、保定市和唐山市等7个环境下种植,各个环境均在收获时调查统计花生侧枝夹角、主茎高、侧枝长、株型指数和扩展半径等5个株型相关性状的表型值。同时,利用SSR、AhTE、SRAP和TRAP等分子标记扫描亲本及群体的基因型用于构建分子遗传连锁图谱。最后结合多年多点的表型数据,采用QTL Icimapping V4.2中的完备区间作图法（inclusive composite interval mapping,ICIM）对7个环境下的株型相关性状进行加性QTL和上位性QTL分析。【结果】构建了一张包含363个多态性位点的分子遗传连锁图谱,所有标记被分配到20条染色体和1个未知连锁群;图谱总长度覆盖全基因组的1 360.38 cM,标记间平均距离为3.75 cM;单个连锁群长度为39.599—101.056 cM,包括4—50个分子标记。共检测到30个与株型相关性状的加性QTL,分布在A04、A05、A06、A08、A09、B02、B09等7条染色体上。其中,5个QTL与侧枝夹角相关,可解释的表型变异（phenotypic variance explained,PVE）为3.48%—11.22%;15个QTL与主茎高相关,PVE为3.58%—10.05%;2个QTL与侧枝长相关,PVE为6.03%—8.56%;4个QTL与株型指数相关,PVE为4.68%—15.08%;4个QTL与扩展半径相关,PVE为3.30%—9.33%。qLBAA05.1、qLBAA05.2、qMSHA04.2和qIOPTA05.1等4个主效QTL,可解释的表型变异分别为11.22%、10.59%、10.23%、10.05%和15.08%。此外,共检测到59对上位性QTL。其中,6对上位性QTL与侧枝长相关,PVE为2.23%—2.78%;50对上位性QTL与株型指数相关,PVE为0.25%—1.44%;3对上位性QTL与扩展半径相关,PVE为7.28%—12.25%。发现3个QTL聚集区,分别为A04染色体的GM1867—AHGS1967区间、A05染色体的me14em5-116—PM418区间和A08染色体的HBAUAh177—AhTE0658区间,涉及侧枝夹角、主茎高、株型指数和扩展半径等4个株型相关性状。【结论】构建了一张包含363个标记位点的分子遗传连锁图谱;获得30个与株型相关性状的加性QTL和59对上位性QTL;发现3个QTL聚集区。
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| 关 键 词： | 花生 株型 QTL 重组自交系 |
| 收稿时间： | 2020-09-23 |
QTL Mapping for Lateral Branch Angle Related Traits of Cultivated Peanut (Arachis hypogaea L.) |
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| MENG XinHao,DENG HongTao,LI Li,CUI ShunLi,Charles Y. CHEN,HOU MingYu,YANG XinLei,LIU LiFeng.QTL Mapping for Lateral Branch Angle Related Traits of Cultivated Peanut (Arachis hypogaea L.)[J].Scientia Agricultura Sinica,2021,54(8):1599-1612. |
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| Authors: | MENG XinHao DENG HongTao LI Li CUI ShunLi Charles Y CHEN HOU MingYu YANG XinLei LIU LiFeng |
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| Institution: | 1College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China for Crop Improvement and Regulation/Key laboratory of Crop Germplasm Resources of Hebei Province, Baoding 071001, Hebei, China2College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, Hebei, China3Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USA |
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| Abstract: | 【Objective】Cultivated peanut (Arachis hypogaea L.) is an important oil and economic crop in worldwide. Plant type is a typical quantitative trait and an important agronomic trait, which is closely related to yield and mechanized harvesting in peanut. Genetic analysis, QTL mapping and identifying tightly linked molecular markers of plant type, will be conducive to the germplasm protection and cultivar identification, and provide an important theoretical basis for the molecular breeding of plant type in cultivated peanut. 【Method】In the present study, a RIL population as research material was established, which consisted of 321 families and derived from Jihua 5 with erect plant type and M130 with prostrate type. Two parents and RIL population were planted at Hainan city, Handan city, Baoding city and Tangshan city during the growing season (May to September) from 2016 to 2018. The phenotypic data of plant type related traits, such as lateral branch angle, main stem height, lateral branch length, index of plant type and extension radius, were investigated at harvesting season under seven environments. Meanwhile, SSR, AhTE, SRAP and TRAP were used to identify genotypic data of parents and RIL that was applied to construct the molecular genetic linkage map. Later, we combined phenotypic data of seven environments, and identified QTLs for plant type related traits using ICIM of QTL Icimapping V4.2. 【Result】A molecular genetic linkage map containing 363 polymorphism sites was constructed, and all markers were assigned to 20 chromosomes and an unknown linkage group. The total length of the map covered 1 360.38 cM of the whole genome, and the average distance between the markers was 3.75 cM. The length of a single linkage group was 39.599-101.056 cM, including 4-50 molecular markers. Subsequently, 30 additive QTLs for plant type related traits were detected by ICIM-ADD method, which were distributed on A04, A05, A06, A08, A09, B02 and B09 chromosomes. Among these QTLs, 5 QTLs for LBA with PVE was 3.48%-11.22%, 15 QTLs for MSH with PVE was 3.58%-10.05%, 2 QTLs for LBL with PVE was 6.03%-8.56%, 4 QTLs for IOPT with PVE was 4.68%-15.08%, 4 QTLs for ER with PVE was 3.30%-9.33%. Of these, qLBAA05.1, qLBAA05.2, qMSHA04.2, and qIOPTA05.1 were main-effect QTLs, explaining 11.22%, 10.59%, 10.23%, 10.05% and 15.08% of the phenotypic variance, respectively. In addition, 59 pairs epistatic QTLs were detected by ICIM-EPI method. Among them, 6 pairs of epistatic QTLs for LBL with PVE were 2.23% to 2.78%, 50 pairs of epistatic QTLs for IOPT with PVE were 0.25% to 1.44%, and 3 pairs of epistatic QTLs for ER with PVE were 7.28% to 12.25%. Finally, we also found 3 QTL clusters for LBA, MSH, IOPT and ER on GM1867-AHGS1967 interval of A04, me14em5-116-PM418 interval of A05 and HBAUAh177-AhTE0658 interval of A08, respectively. 【Conclusion】In brief, we constructed a molecular genetic linkage map containing 363 loci, and identified 30 additive QTLs and 59 pairs of epistatic QTLs for plant type related traits, and found 3 QTL clusters. |
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| Keywords: | peanut plant type QTL RILs |
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