| 贵州魔芋软腐病菌多基因分子鉴定及其致病力分析 |
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| 引用本文： | 黄露,周兵正,安星宇,王莉爽,姚令,陈小均,何海永,吴石平.贵州魔芋软腐病菌多基因分子鉴定及其致病力分析[J].植物保护,2023,49(4):115-124. |
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| 作者姓名： | 黄露 周兵正 安星宇 王莉爽 姚令 陈小均 何海永 吴石平 |
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| 作者单位： | 1. 贵州省农业科学院植物保护研究所, 贵阳550009; 2. 贵州医科大学, 贵阳550025; 3. 贵州大学, 贵阳550025 |
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| 基金项目： | 贵州省农业科学院青年基金(黔农科院青年基金[2018]96号) |
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| 摘 要： | 为明确贵州魔芋软腐病菌种类?致病力及分布特点, 采用组织分离法对贵州主要魔芋种植区软腐病样进行病原菌分离, 对icdA, mdh, mtlD, proA, rpoS 等5个管家基因进行了扩增?序列测定, 分别用单基因和多基因联合系统发育树对病菌进行鉴定, 同时采用组织块接种方法测定了不同菌株的致病力?通过组织分离法共分离魔芋软腐病菌株47株; 采用5个管家基因进行分子鉴定, 将病菌分别鉴定为海芋果胶杆菌Pectobacterium aroidearum?胡萝卜果胶杆菌Pectobacterium carotovorum和方中达迪基氏菌Dickeya fangzhongdai 3个种, 其中海芋果胶杆菌P.aroidearum为贵州魔芋软腐病主要致病菌, 占分离菌株的70%, 广泛分布在多个地区; 其次为方中达迪基氏菌D. fangzhongdai, 占分离菌株的28%, 也普遍存在于贵州各魔芋种植区; 胡萝卜果胶杆菌P. carotovorum最少, 占分离菌株的2%?致病力测定结果表明, 菌株间致病力存在一定的差异, 其中海芋果胶杆菌不同菌株之间致病力差异较大, 低?中?高致病力菌株都有, 方中达迪基氏菌差异较小, 仅有中?高致病力菌株?本研究确定了贵州魔芋软腐病菌种类?致病力及在贵州的分布特点, 首次报道了海芋果胶杆菌?方中达迪基氏菌是贵州魔芋软腐病的主要病原菌, 进一步加深了对魔芋软腐病及其发生流行的认识, 为软腐病的科学防控提供了科学依据?
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| 关 键 词： | 魔芋 软腐病 病原鉴定 致病力 |
| 收稿时间： | 2022/6/14 0:00:00 |
| 修稿时间： | 2022/8/11 0:00:00 |
Molecular identification based on multiple genes and pathogenicity analysis of soft rot pathogens of Amorphophallus konjac in Guizhou |
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| HUANG Lu,ZHOU Bingzheng,AN Xingyu,WANG Lishuang,YAO Ling,CHEN Xiaojun,HE Haiyong,WU Shiping.Molecular identification based on multiple genes and pathogenicity analysis of soft rot pathogens of Amorphophallus konjac in Guizhou[J].Plant Protection,2023,49(4):115-124. |
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| Authors: | HUANG Lu ZHOU Bingzheng AN Xingyu WANG Lishuang YAO Ling CHEN Xiaojun HE Haiyong WU Shiping |
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| Institution: | 1. Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China; 2. Guizhou Medical University, Guiyang550025, China; 3. Guizhou University, Guiyang 550025, China |
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| Abstract: | In order to identify the pathogens causing soft rot of konjac and investigate their pathogenicity and distribution in Guizhou, the pathogens were isolated from infected samples in main konjac planting areas, and five housekeeping genes (icdA, mdh, mtlD, proA and rpoS) were amplified and sequenced. Molecular identification of the pathogens was performed by single gene and multi-gene phylogeny, and the pathogenicity of the pathogens were tested by tissue block inoculation. Forty-seven strains were isolated from soft rot samples in Guizhou and the strains were identified as Pectobacterium aroidearum, P. carotovorum and Dickeya fangzhongdai by molecular identification based on five housekeeping genes. P. aroidearum was the main pathogen with the isolation frequency of 70%, which was widely distributed in many regions of Guizhou, followed by D. fangzhongdai with the frequency of 28%. P. carotovorum was the least distributed with the frequency of 2%. The results of pathogenicity test reveal that there was obvious difference in the pathogenicity of P. aroidearum strains, including low, medium and high pathogenic strains, while D. fangzhongdai strains showed less variation, with only medium and high pathogenic strains. This study identified the species, pathogenicity, distribution of soft rot pathogens. It is the first report of P. aroidearum, D. fangzhongdai being the main pathogens of konjac soft rot in Guizhou. It deepens our understanding of the pathogen and its occurrence epidemic of soft rot of konjac and provides a scientific basis for scientific prevention and control of soft rot disease. |
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| Keywords: | Amorphophallus konjac soft rot pathogen identification pathogenicity |
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