| 枯草芽孢杆菌添加剂对生物絮团菌群结构和营养成分的影响 |
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| 引用本文： | 贺希,罗国芝,谭洪新.枯草芽孢杆菌添加剂对生物絮团菌群结构和营养成分的影响[J].上海海洋大学学报,2022,31(4):873-882. |
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| 作者姓名： | 贺希 罗国芝 谭洪新 |
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| 作者单位： | 上海海洋大学,上海海洋大学,上海海洋大学 |
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| 基金项目： | 上海市科学技术委员会资助项目（14320501900） |
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| 摘 要： | 为探究枯草芽孢杆菌添加剂对生物絮团菌群结构、添加菌在絮团上的富集效果、絮团营养以及系统构建过程水质的影响。生物絮凝系统构建过程中，枯草芽孢杆菌的添加剂量分别为A组106 CFU/mL、B组105 CFU/mL、C组104 CFU/mL和D组0 CFU/mL，添加周期为3 天/次。结果表明：生物絮凝系统构建过程中，枯草芽孢杆菌添加剂量对絮团总悬浮颗粒物(Total suspended solids, TSS)、5 分钟内絮团沉降体积(Floc Volume, FV)影响显著(P＜0.05)；对三态氮浓度变化趋势影响较明显；对絮团氨基酸含量、粗蛋白含量、絮团碳氮比影响显著(P＜0.05)，对絮团粗脂肪，粗灰分含量影响不显著(P＞0.05)；添加枯草芽孢杆菌絮团中氨基酸、粗蛋白含量高于不添加枯草芽孢杆菌组；枯草芽孢杆菌添加剂量对絮团门、属水平菌群差异物种相对丰度有显著影响(P＜0.05)，增加枯草芽孢杆菌添加剂浓度，絮团中芽孢杆菌浓度也随之升高。综上所述，枯草芽孢杆菌能在生物絮团上得到有效富集，在生物絮凝系统构建过程中添加枯草芽孢杆菌能提高絮团粗蛋白和氨基酸含量，有利于为养殖对象提供蛋白源。
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| 关 键 词： | 枯草芽孢杆菌 添加剂量 絮团营养 菌群结构 |
| 收稿时间： | 2021/4/21 0:00:00 |
| 修稿时间： | 2021/6/15 0:00:00 |
Effect of adding Bacillus subtilis on the microbial community structure and nutrition of bioflocs |
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| HE Xi,LUO Guozhi,TAN Hongxin.Effect of adding Bacillus subtilis on the microbial community structure and nutrition of bioflocs[J].Journal of Shanghai Ocean University,2022,31(4):873-882. |
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| Authors: | HE Xi LUO Guozhi TAN Hongxin |
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| Institution: | Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to explore the effect of adding Bacillus subtilis on the microbial community structure, nutrition of bioflocs and water quality in the process of system construction, the biofloc technology-aquaculture system was completed, the additive concentration of B. subtilis was group A, 106 CFU/mL, group B,105 CFU/mL, group C, 104 CFU/mL, group D, 0 CFU/mL. The experimental results showed that:The concentration of B. subtilis had significant effect on the concentration of TSS and FV-30; The concentration of B. subtilis had a significant effect on the amino acid content and C/N of the floc; The concentration of B. subtilis had no significant effect on crude lipid and ash content; The amino acid and crude protein contents of adding B. subtilis groups were higher than those of the non-added group; The additive of B. subtilis had a significant effect on the relative abundance at phylum and genus level of bacteria; and increasing the concentration of B. subtilis additive led to an increase in the concentration of Bacillus in the biofloc. B. subtilis can be effectively enriched on bioflocs. The addition of B. subtilis can effectively improve the nutrient content of bioflocs, which is conducive to providing protein source for aquaculture. |
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| Keywords: | Bacillus subtilis Additive amount microbial communities Biofloc nutrition |
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