| 雌性三角帆蚌外鳃组织形态周年变化及低氧胁迫对外鳃组织形态和酶活性影响 |
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| 引用本文： | 郭思鹏,汪桂玲,白志毅,孙田洋.雌性三角帆蚌外鳃组织形态周年变化及低氧胁迫对外鳃组织形态和酶活性影响[J].上海海洋大学学报,2022,31(4):865-872. |
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| 作者姓名： | 郭思鹏 汪桂玲 白志毅 孙田洋 |
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| 作者单位： | 上海海洋大学 水产与生命学院,上海海洋大学 水产与生命学院,上海海洋大学 水产与生命学院,上海海洋大学 水产与生命学院 |
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| 基金项目： | 国家重点研发计划（2018YFD0901406）；现代农业产业技术体系建设专项（CARS-49）；江苏省苏北科技专项（SZ-SQ2019045） |
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| 摘 要： | 雌性三角帆蚌的外鳃不仅是呼吸器官，而且在繁育时期行使胚胎发育的育儿囊功能，繁育期鳃腔充满早期胚胎，呼吸效率降低。通过石蜡切片观察雌性三角帆蚌外鳃组织形态周年变化，并探究低氧环境对雌性三角帆蚌外鳃组织形态及酶活性的影响。结果表明，雌性三角帆蚌外鳃鳃瓣1月份最薄，至5月份增至最厚，较1月份显著增厚173.86%，随后鳃瓣逐渐变薄，其鳃小腔宽度随鳃瓣厚度增厚也显著增大，至6月增至最宽较1月份显著增宽171.22%，随后鳃小腔逐渐变窄；且3~6月在雌性三角帆蚌外鳃发育为育儿囊，其中发现大量早期胚胎。在低氧环境下，乳酸脱氢酶（LDH）活性在1~5天逐渐升高，第5天达到最大值，较对照组显著提升131.1%，5~9天缓慢下降；超氧化物歧化酶（SOD）活性在1~7天逐渐下降，在第7天达到最小值，较对照组显著降低40.53%，7~9天缓慢上升；过氧化氢酶（CAT）活性在1~7天逐渐上升，7~9天无明显变化，第7天活性最高，较对照组显著提升91.34%，琥珀酸脱氢酶（SDH）活性在1~9天呈下降趋势，最低值出现在第9天，较最对照组显著降低68.9%；低氧胁迫5天后，外鳃侧纤毛较对照组增宽了79.58%，外鳃小水管较对照组增宽了248.73%。本研究分别为怀卵期雌性三角帆蚌外鳃组织结构变化和低溶氧对雌性三角帆蚌外鳃组织结构和酶活性的影响提供了基础资料，为提升三角帆蚌繁育技术提供了理论依据。
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| 关 键 词： | 三角帆蚌 外鳃 育儿囊 低氧 酶活性 |
| 收稿时间： | 2021/4/10 0:00:00 |
| 修稿时间： | 2021/6/22 0:00:00 |
Annual changes of outer gill tissue morphology and effects of hypoxia stress on outer gill tissue morphology and enzyme activities in female Hyriopsis cumingii |
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| GUO Sipeng,WANG Guiling,BAI Zhiyi,SUN Tianyang.Annual changes of outer gill tissue morphology and effects of hypoxia stress on outer gill tissue morphology and enzyme activities in female Hyriopsis cumingii[J].Journal of Shanghai Ocean University,2022,31(4):865-872. |
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| Authors: | GUO Sipeng WANG Guiling BAI Zhiyi SUN Tianyang |
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| Institution: | Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University |
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| Abstract: | The outer gill of female Hyriopsis cumingii is not only a respiratory organ, but also performs the function of embryo sac during the reproduction period. During the reproduction period, the gill cavity is full of early embryos, and the respiratory efficiency is reduced. The annual changes of the outer gill tissue morphology of female Hyriopsis cumingii were observed by paraffin section, and the effects of hypoxia environment on the outer gill tissue morphology and enzyme activities of female Hyriopsis cumingii were explored. The results showed that the outer gill of female Hyriopsis cumingii was the thinnest in January, and increased to the thickest in May, which was 173.86% thicker than that in January. Then, the gill gradually became thinner, and the width of the gill cavity also increased significantly with the thickness of the gill, and increased to the widest in June, which was 171.22% wider than that in January, and then the gill cavity gradually narrowed There are a large number of early embryos. In hypoxia environment, the activity of lactate dehydrogenase (LDH) increased gradually in 1-5 days, reached the maximum value in 5 days, increased 131.1% compared with the control group, and decreased slowly in 5-9 days; the activity of superoxide dismutase (SOD) decreased gradually in 1-7 days, reached the minimum value in 7 days, decreased 40.53% compared with the control group, and increased slowly in 7-9 days; the activity of catalase (CAT) increased gradually in 1-7 days The activity of succinate dehydrogenase (SDH) decreased from 1 to 9 days, and the lowest value appeared on the 9th day, which was 68.9% lower than that of the control group. After 5 days of hypoxia stress, the cilia on the lateral side of the outer gill were 79.58% wider than that of the control group, and the aqueduct of the outer gill were 248.73% wider than that of the control group. This study provided basic data for the changes of outer gill tissue structure of female Hyriopsis cumingii and the effects of low dissolved oxygen on the tissue structure and enzyme activities of outer gill of female Hyriopsis cumingii, and provided theoretical basis for improving the breeding technology of Hyriopsis cumingii. |
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| Keywords: | Hyriopsis cumingii outer gill nursery sac hypoxia enzyme activity |
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