| 生猪伪狂犬病毒gB抗体与中和抗体的相关性分析 |
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| 引用本文： | 杨涛涛,刘崇灵,刘晓波,赵墩,余兴龙.生猪伪狂犬病毒gB抗体与中和抗体的相关性分析[J].湖南农业大学学报(自然科学版),2015,41(3). |
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| 作者姓名： | 杨涛涛 刘崇灵 刘晓波 赵墩 余兴龙 |
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| 作者单位： | 湖南农业大学动物医学院,湖南长沙,410128 |
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| 基金项目： | 长沙市科技成果产业化重点项目(K1308135–31) |
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| 摘 要： | 为探索生猪伪狂犬病毒gB–ELISA抗体水平的高低与中和抗体效价的相关性,运用酶联免疫吸附测定(ELISA)法对177份生猪血清进行了伪狂犬病毒gB抗体的检测,同时,用中和试验对上述血清样品进行了伪狂犬病毒中和抗体的测定。试验结果通过生物统计学分析,gE抗体阴性血清的g B抗体平均S/P值和中和抗体效价(SN效价)倒数平均值的相关系数为0.926,说明g B免疫抗体S/P值与其中和抗体效价有很好的相关性。中和抗体水平的高低一般与免疫保护力呈正相关,研究结果表明gB–ELISA抗体水平可以用来评估生猪对当前流行PRV毒株的相对免疫保护力。
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| 关 键 词： | 猪 伪狂犬病毒 gB抗体 中和抗体 酶联免疫吸附测定 |
Correlation analysis of PRV gB-antibody and neutralizing antibody |
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| Yang Taotao,Liu Chongling,Liu Xiaobo,Zhao Dun,Yu Xinglong.Correlation analysis of PRV gB-antibody and neutralizing antibody[J].Journal of Hunan Agricultural University,2015,41(3). |
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| Authors: | Yang Taotao Liu Chongling Liu Xiaobo Zhao Dun Yu Xinglong |
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| Abstract: | The PRV gB-antibody value from 177 serum samples were detected using the approach of ELISA and the PRV neutralizing antibody titer in the same samples were measured through neutralization test to discuss their correlationship. The result from biostatistics showed that the correlation coefficient between mean S/P value of gB-antibody and neutralizing antibody titer in negative serum of gE-antibody was 0.926, which indicated that S/P value of gB-antibody and neutralizing antibody titer in immune serums have a good correlation. Generally speaking, neutralizing antibody titer had a positive correlation with protective immunity, which showed that S/P value of gB-antibody could act as an index to evaluate the porcine protective immunity for the current epidemic PRV. |
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| Keywords: | pig pseudorabiesvirus(PRV) gB-antibody neutralizing antibody enzyme-linked immunosorbent assay (ELISA) |
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