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| 抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲 | |
| 引用本文： | 李磊,薛芗,左示敏,陈宗祥,张亚芳,李前前,朱俊凯,马玉银,潘学彪.抑制OsAGO1a基因的表达导致水稻叶片近轴面卷曲[J].中国水稻科学,2013,27(3):223-230. |
| 作者姓名： | 李磊 薛芗 左示敏 陈宗祥 张亚芳 李前前 朱俊凯 马玉银 潘学彪 |
| 作者单位： | 1. 扬州大学农学院,江苏扬州,225009 2. 江苏里下河地区农业科学研究所,江苏扬州,225007 3. 扬州职业大学生化工程系,江苏扬州,225009 |
| 基金项目： | 国家自然科学基金资助项目，江苏省自然科学基金资助项目 |
| 摘 要： | AGO蛋白是RNA诱导沉默复合体的核心元件，在植物生长发育过程中发挥着重要作用。通过分别特异性地扩增OsAGO1a基因中第2外显子和第11~12外显子的片段，构建了两个干涉载体OsAGO1aI 1E和OsAGO1aI 2E。利用农杆菌介导法转化水稻品种日本晴，抑制OsAGO1a基因的表达，借此研究OsAGO1a的功能及其对水稻生长发育的影响。结果表明, 抑制OsAGO1a的表达导致叶片的近轴面卷曲而对其他主要农艺性状的影响不大。 |
| 关 键 词： | 水稻 OsAGO1a 极性发育 卷叶 农艺性状 |
| 收稿时间： | 2012-12-13; |
Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice |
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| LI Lei,XUE Xiang,ZUO Shi min,CHEN Zong xiang,ZHANG Ya fang,LI Qian qian,ZHU Jun kai,MA Yu yin,PAN Xue biao,PAN Cun hong.Suppressed Expression of OsAGO1a Leads to Adaxial Leaf Rolling in Rice[J].Chinese Journal of Rice Science,2013,27(3):223-230. | |
| Authors: | LI Lei XUE Xiang ZUO Shi min CHEN Zong xiang ZHANG Ya fang LI Qian qian ZHU Jun kai MA Yu yin PAN Xue biao PAN Cun hong |
| Institution: | Agricultural College, Yangzhou University, Yangzhou 225009, China; Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225007, China; Department of Biochemical Engineering, Yangzhou Polytechnic College, Yangzhou 225009, China; #These authors contribute equally to this paper; |
| Abstract: | Argonaute (AGO) protein is the core effectors in RNA induced silencing complexes (RISCs), playing an important role in plant development. The 2nd and the 11th-12th exon fragments of OsAGO1awere amplified independently to make two RNAi constructs: OsAGO1aI 1E and OsAGO1aI 2E. To clarify the function of OsAGO1a in rice development, the expression of OsAGO1a has been suppressed by introducing RNAi constructs to Nipponbare through Agrobacterium mediated transformation. The result showed that the reduced expression of OsAGO1a lead to adaxial leaf rolling without affecting main agronomic traits. |
| Keywords: | rice OsAGO1a polarity development rolled leaf agronomic traits |
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