Production and characterization of fibroin hydrogel using waste silk fibers |
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| Authors: | Natalia Marchesan Bexiga Antonio Carlos Bloise Mariana Agostini de Moraes Attilio Converti Marisa Masumi Beppu Bronislaw Polakiewicz |
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| Institution: | 1.Laboratory of Applied Organic Synthesis, Department of Biochemical and Pharmaceutical Technology,University of S?o Paulo,S?o Paulo,Brazil;2.Laboratory of Microrheology and Molecular Physiology, Department of General Physics, Institute of Physics,University of S?o Paulo,S?o Paulo,Brazil;3.Department of Exact and Earth Sciences, Engineering Area,Federal University of S?o Paulo,Diadema,Brazil;4.Department of Civil, Chemical and Environmental Engineering,University of Genoa, Pole of Chemical Engineering,Genoa,Italy;5.Engineering and Chemist Products Laboratory, Department of Materials and Bioprocess Engineering, School of Chemical Engineering,University of Campinas,Campinas,Brazil |
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| Abstract: | The use of natural resources, especially processing wastes, as low cost and environmentally friendly alternative aiming high value-added applications is a subject of broad interest. Since the Brazilian silk production annually generates a large amount of waste during the silk fibers processing, this work explores the preparation and characterization of silk fibroin hydrogels using spinning waste silk fibers from textile processing and the processed ones. Hydrogels were obtained directly by dialyzing silk fibroin solutions against frequent changes of water until the gelation point and then lyophilized and characterized in terms of their morphology, crystallinity, thermal resistance and secondary structure. X-ray diffraction analysis revealed the presence of β-sheet conformation related to sol-gel transition. FT-IR spectra indicated the coexistence of random coil (silk I) and β-sheet (silk II) structures, with predominance of β-sheet conformation for hydrogels from processed silk fibers. From thermogravimetric analysis the presence of β-sheet secondary conformation was demonstrated by a degradation peak around 292 °C for both hydrogels. Freeze-dried hydrogels presented sheet or leaf like morphology and no significant change was observed among the hydrogels from waste silk fibers and processed ones. These characteristics suggest that silk fibroin hydrogels prepared from spinning waste silk fibers and obtained directly by dialysis can be potential candidates for biomaterials application, such as drug delivery systems and for wound dressings. |
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