Mapping of the leaf rust resistance gene <Emphasis Type="Italic">Lr38</Emphasis> on wheat chromosome arm 6DL using SSR markers |
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| Authors: | S A Mebrate E C Oerke H W Dehne K Pillen |
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| Institution: | 1.Institute of Crop Science and Resource Conservation, Phytomedicine,University of Bonn,Bonn,Germany;2.Debre Zeit Agricultural Research Center,Debre Zeit,Ethiopia;3.Institute of Crop Science and Resource Conservation, Crop Genetics and Biotechnology,University of Bonn,Bonn,Germany;4.Max-Planck-Institute for Plant Breeding Research,Barley Genetics Research Group,Cologne,Germany |
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| Abstract: | Leaf rust caused by the fungus Puccinia triticina is one of the most important diseases of wheat (Triticum aestivum) worldwide. The use of resistant wheat cultivars is considered the most economical and environment-friendly approach in controlling
the disease. The Lr38 gene, introgressed from Agropyron intermedium, confers a stable seedling and adult plant resistance against multiple isolates tested in Europe. In the present study, 94
F2 plants resulting from a cross made between the resistant Thatcher-derived near-isogenic line (NIL) RL6097, and the susceptible
Ethiopian wheat cultivar Kubsa were used to map the Thatcher Lr38 locus in wheat using simple sequence repeat (SSR) markers. Out of 54 markers tested, 15 SSRs were polymorphic between the
two parents and subsequently genotyped in the population. The P. triticina isolate DZ7-24 (race FGJTJ), discriminating Lr38 resistant and susceptible plants, was used to inoculate seedlings of the two parents and the segregating population. The
SSR markers Xwmc773 and Xbarc273 flanked the Lr38 locus at a distance of 6.1 and 7.9 cM, respectively, to the proximal end of wheat chromosome arm 6DL. The SSR markers Xcfd5 and Xcfd60 both flanked the locus at a distance of 22.1 cM to the distal end of 6DL. In future, these SSR markers can be used by wheat
breeders and pathologists for marker assisted selection (MAS) of Lr38-mediated leaf rust resistance in wheat. |
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| Keywords: | Agropyron intermedium Triticum aestivum Puccinia triticina Leaf rust resistance SSR markers |
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