| 绵羊肺炎支原体感染山羊病例的实验室诊断 |
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| 引用本文： | 郭小江,洪猛,程振涛,张双翔,周碧君,文明.绵羊肺炎支原体感染山羊病例的实验室诊断[J].贵州农业科学,2012(3):147-149. |
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| 作者姓名： | 郭小江 洪猛 程振涛 张双翔 周碧君 文明 |
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| 作者单位： | 贵州大学动物科学学院;铜仁市动物疫病预防控制中心;贵州省动物疫病研究室 |
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| 基金项目： | 贵州省科技厅项目“贵州省动物疫病与兽医公共卫生重点实验室培育”[黔科合计Z字(2011)4008];铜仁市畜牧兽医局科技项目“山羊传染性胸膜肺炎综合防治技术研究”[铜地牧发(2011)64];贵州大学人才基金“羊传染性胸膜肺炎病原分离鉴定与分子特征研究”[贵大人基合字(2010)35] |
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| 摘 要： | 为确诊铜仁地区山羊肺炎病例病原,开展了山羊肺炎的发病情况调查、临床症状与病理变化观察和血清抗体与病原核酸检测等实验室诊断。结果表明:该病以2～3月龄山羊最为易感,秋冬两季多发;临床症状多为呼吸系统症状,病理变化主要是纤维素性胸膜肺炎;绵羊肺炎支原体抗体阳性率为69.6%(78/112),病原核酸检出率为64.3%(9/14);丝状支原体山羊亚种抗体阳性率为8.0%(9/112),未检出其病原核酸。绵羊肺炎支原体是引起铜仁地区山羊支原体肺炎的主要病原。
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| 关 键 词： | 山羊肺炎支原体 绵羊肺炎支原体 血清抗体 病原核酸 |
Laboratory Diagnosis of Goats Infected by Mycoplasma ovipneumoniae |
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| GUO Xiao-jiang,HONG Meng,CHENG Zhen-tao,ZHANG Shuang-xiang,ZHOU Bi-jun,WEN Ming.Laboratory Diagnosis of Goats Infected by Mycoplasma ovipneumoniae[J].Guizhou Agricultural Sciences,2012(3):147-149. |
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| Authors: | GUO Xiao-jiang HONG Meng CHENG Zhen-tao ZHANG Shuang-xiang ZHOU Bi-jun WEN Ming |
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| Institution: | 1,3 (1.Animal Science College of Guizhou University,Guiyang,Guizhou 550025;2.Tongren Center for Animal Disease Prevention and Control,Tongren,Guizhou 550025;3.Guizhou Institute for Animal Disease,Guiyang,Guizhou 550025,China) |
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| Abstract: | The survey of incidence,observation of clinical symptoms and pathological changes,detection of serum antibodies and pathogen nucleic acid were conducted in laboratory to clarify the pathogen of pneumonia cases.Results: 2～3 months old goats were susceptible to the disease and often occurred in autumn and winter.The clinical symptoms of the disease were mainly in respiratory system,and pathological changes were mainly cellulose pleural pneumonia.The positive rate of antibodies to M.ovipneumoniea was 69.6%(78/112) in sick goats,and the detected rates of the specific DNA fragment of this pathogen was 64.3%(9/14) in the lung tissues of the sick goats.The positive rate of antibodies to M.mycoides subsp.capri was 8.0%(9/112) and the specific DNA fragment was not detected.It was concluded that M.ovipneumoniea was the major causative agent of CMP in Tongren Region. |
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| Keywords: | Mycoplasma capripneumonia Mycoplasma ovipneumoniae serum antibody pathogen nucleic acid |
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