| 白腐真菌F9产漆酶发酵条件的优化 |
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| 引用本文： | 艾良,朱启忠,李文静,宋炳红.白腐真菌F9产漆酶发酵条件的优化[J].北方园艺,2010(4):144-146. |
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| 作者姓名： | 艾良 朱启忠 李文静 宋炳红 |
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| 作者单位： | 山东大学威海分校,海洋学院,山东,威海,264209;山东大学威海分校,海洋学院,山东,威海,264209;山东大学威海分校,海洋学院,山东,威海,264209;山东大学威海分校,海洋学院,山东,威海,264209 |
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| 摘 要： | 采用液体摇瓶培养方法,探讨了碳源、氮源、pH值、培养温度等各种因素对白腐真菌分泌漆酶能力的影响,采用正交试验对主要的影响因素进行了优化。优化的培养条件为:马铃薯200 g/L,葡萄糖20 g/L,酵母膏5 g/L,MgSO41.5 g/L,VB10.1 g/L,ZnSO40.05 g/L,MnSO40.05g/L,KH2PO43.0 g/L,pH 6,120 r/min,28℃发酵到10 d左右粗酶液酶活达到109.1μ/mL。
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| 关 键 词： | 漆酶 培养条件 酶活 |
Studies on Fermentation Conditions for Laccase Production from White-Rot Fungi F9 |
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| AI Liang,ZHU Qi-zhong,LI Wen-jing,SONG Bing-hong.Studies on Fermentation Conditions for Laccase Production from White-Rot Fungi F9[J].Northern Horticulture,2010(4):144-146. |
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| Authors: | AI Liang ZHU Qi-zhong LI Wen-jing SONG Bing-hong |
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| Institution: | Marine College of Shandong University at Weihai;Weihai;Shandong 264209 |
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| Abstract: | The main factors affecting laccase production in liquid shake-flask fermentation were studied.The effects of carbon source,nitrogen source,pH value culture,culture temperature on producing laccase were studied.The optimization of fermentation conditions were conducted according to the orthogonal experiment.The optimal fermentation conditions for laccase production were as follows: potato 200 g/L,yeast paste 5 g/L,MgSO4 1.5 g/L,vitamin B1 0.1 g/L,ZnSO4 0.05 g/L,MnSO4 0.05 g/L,KH2PO4 3.0 g/L,pH 6,and incubati... |
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| Keywords: | laccase culture condition enzyme activity |
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